The purpose of this study is to evaluate the absorption of dietary supplement of vitamin D3 (cholecalciferol), through the variation of 25 (OH) D levels, as the fat content of the meal associated with the administration of the supplement.

Clinical randomized controlled double-blind study with healthy subjects to investigate the effect of low and high fat of meal on absorption of vitamin D supplement. Participants were randomly in two groups according to sex and BMI.

In the same date, each group of participants received a meal and dietary supplement of vitamin D3 (cholecalciferol) after the first blood sample and a urine sample. Others samples were collected in the seventh and fourteenth day after intake the supplement.

Inclusion Criteria:

• Residents of the hospital clinics of Porto Alegre

Exclusion Criteria:

• Don't drink milk
• Obesity
• Malnutrition
• Liver disease
• Kidney disease or diabetes
• Use of dietary supplements containing calcium and vitamin D
• Medications
• Anticonvulsants
• Barbiturates, or steroids.
• Having performed a journey the last four months to locations near the Earth's equatorial plane, due to sun exposure that may have occurred during this period, or vacations planned during the study period.