Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients.

To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.

Inclusion Criteria:

• Age 18-50 years
• No physical limitation to perform ischemic exercise
• Informed consent

Exclusion Criteria:

• Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l)
• hyperlipidemia (random total cholesterol > 6.5 mmol/l)
• Hypertension (supine SBP/DBP > 140/90 mmHg at screening)
• Any cardiovascular disease
• Drug abuse
• Concomitant chronic use of medication
• Administration of radioactivity in research setting during the last 5 years
• Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.