Inclusion Criteria:

• Adult men or women (18-50 years)
• Diagnosis of MASLD (grade 1 or 2 of steatosis confirmed by ultrasound)
• Body mass index (BMI) = 25-34.9 kg/m²
• Providing written informed consent

Exclusion Criteria:

• Pregnancy, lactation, or plans to get pregnant during the next three months.
• Liver disease (viral hepatitis, autoimmune liver disease, cirrhosis, drug-induced hepatotoxicity, or alcoholic fatty liver disease), heart or renal failure, kidney stones, any neoplasia, inflammatory disease, hypothyroidism, hypercortisolism, or hypertension
• Taking drugs affecting glucose or lipid metabolism, folate supplements, anti-obesity medications, weight-loss diets, or dietary supplements
• Lifestyle factors known to impact folate status (current smoking, alcohol intake, recreational drug use)
• Pre-existing conditions affecting folate status (malabsorptive or inflammatory bowel diseases, active celiac disease, gastric bypass surgery, atrophic gastritis, epilepsy, advanced liver disease, kidney dialysis, type 1 or 2 diabetes mellitus, or sickle cell trait/anemia)
• Medications that interfere with B-vitamin metabolism (chloramphenicol, methotrexate, metformin, sulfasalazine, phenobarbital, phenytoin, primidone, triamterene, barbiturates)