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This study investigated whether exercising in cold environments affects appetite, energy intake, and food preference compared with exercising in thermoneutral conditions. Eleven healthy males completed five laboratory trials as part of a crossover study: a control trial and four exercise trials performed in cold-water (16°C), thermoneutral water (35°C), cold-ambient air (6°C), and thermoneutral ambient air (26°C). Appetite responses were assessed throughout each trial, and participants were provided with an ad libitum meal after exercise to measure energy intake. Food preference was assessed using the Leeds Food Preference Questionnaire. The study aimed to determine whether environmental temperature and exercise medium influence acute post-exercise eating behaviour.
Exercise performed in cold environments may influence thermoregulatory responses and subsequent eating behaviour differently compared with exercise performed under thermoneutral conditions. Previous research suggests that cold exposure may increase post-exercise energy intake; however, the influence of environmental temperature and exercise medium (water versus air) on appetite regulation and food-related behaviour remains unclear.
This study used a randomised repeated-measures crossover design to investigate the effects of cold and thermoneutral exercise conditions on appetite, energy intake, and food preference in healthy males.
Eleven healthy males completed five laboratory trials: a no-exercise control condition and four experimental exercise conditions consisting of 30 minutes of arm-leg rowing exercise performed in cold-water (16°C), thermoneutral water (35°C), cold-ambient air (6°C), and thermoneutral ambient air (26°C). Trials were separated by a minimum seven-day washout period.
Appetite responses were assessed using visual analogue scales throughout each trial. Following exercise, participants consumed an ad libitum meal to assess acute post-exercise energy intake. Food preference responses, including food appeal and preference measures, were assessed using the Leeds Food Preference Questionnaire.
The primary objective was to determine whether environmental temperature and exercise medium influence post-exercise energy intake. Secondary objectives were to examine effects on subjective appetite and food preference measures.
This study provides insight into how exercising in different thermal environments may influence immediate eating behaviour following exercise.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control Condition | Active Comparator | Resting control condition with no exercise performed. Participants completed the same standardised laboratory procedures and outcome assessments under resting conditions. |
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| Cold-Water Exercise | Experimental | Participants completed 30 minutes of arm-leg rowing exercise performed in cold water at 16°C. |
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| Thermoneutral Water Exercise | Experimental | Participants completed 30 minutes of arm-leg rowing exercise performed in thermoneutral water at 35°C. |
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| Cold-Ambient Air Exercise | Experimental | Participants completed 30 minutes of arm-leg rowing exercise performed in cold ambient air at 6°C. |
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| Thermoneutral Ambient Air Exercise | Experimental | Participants completed 30 minutes of arm-leg rowing exercise performed in thermoneutral ambient air at 26°C. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Control Condition | Other | Resting control condition with no exercise exposure. |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Post-exercise ad libitum energy intake | Energy intake consumed during an ad libitum homogeneous pasta meal provided after each 30-minute experimental/control condition. The mass of food consumed was determined by subtracting the mass of food remaining (including leftovers on the plate and cutlery). Absolute energy intake was calculated using the nutritional information provided by the food manufacturer. | 30 minutes after completion of the 30-minute experimental/control condition. |
| Subjective appetite responses | Subjective appetite responses including hunger, fullness, satisfaction, and prospective food consumption assessed using a 100-mm Visual Analogue Scale (VAS) ranging from 0 mm (not at all) to 100 mm (extremely). Higher scores indicate greater subjective appetite sensations. | Before and after breakfast, before the 30-minute experimental/control condition, during the 30-minute experimental/control condition, and before and after the ad libitum meal. |
| Food preference and food reward responses | Food preference and reward responses assessed using the Leeds Food Preference Questionnaire (LFPQ). Outcomes include explicit liking, explicit wanting, implicit wanting, and fat and sweet appeal bias scores. Higher scores indicate greater preference or reward response for the respective food category (fat or sweet). | 15 minutes after completion of each 30-minute experimental/control trial and 15-minutes before the ad libitum meal. |
| Measure | Description | Time Frame |
|---|---|---|
| Core body temperature | Changes in core body temperature measured continuously using a rectal thermistor during and after each experimental/control condition. | During the 30-minute experimental/control condition and during the 60-minute post-exposure recovery period. |
| Energy expenditure |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Centre for Physical Activity, Sport and Exercise Sciences, Research Laboratory, Coventry University | Coventry | CV1 5FB | United Kingdom |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 36375602 | Background | Grigg MJ, Thake CD, Allgrove JE, King JA, Thackray AE, Stensel DJ, Owen A, Broom DR. Influence of water-based exercise on energy intake, appetite, and appetite-related hormones in adults: A systematic review and meta-analysis. Appetite. 2023 Jan 1;180:106375. doi: 10.1016/j.appet.2022.106375. Epub 2022 Nov 12. | |
| 40221072 | Background |
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Individual participant data will not be shared. Study results will be reported in aggregate form through publications and presentations. Data will be retained securely in accordance with Coventry University research governance and data protection requirements.
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Participants completed five laboratory trials in a repeated-measures crossover design. All participants first completed a control condition, followed by four experimental exercise conditions completed in a randomised order: cold-water exercise (16°C), thermoneutral water exercise (35°C), cold-ambient air exercise (6°C), and thermoneutral ambient air exercise (26°C). Each participant completed all conditions, with trials separated by a minimum seven-day washout period.
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| Cold-Water Exercise |
| Other |
Arm-leg rowing exercise performed in 16°C water. |
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| Thermoneutral Water Exercise | Other | Arm-leg rowing exercise performed in 35°C water. |
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| Cold-Ambient Air Exercise | Other | Arm-leg rowing exercise performed in 6°C ambient air. |
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| Thermoneutral Ambient Air Exercise | Other | Arm-leg rowing exercise performed in 26°C ambient air. |
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Energy expenditure during the exercise/control condition estimated from breath-by-breath oxygen consumption and carbon dioxide production using indirect calorimetry. |
| During the 30-minute experimental/control condition. |
| Skin temperature and mean body temperature responses | Skin temperature was measured at four sites (chest, bicep, thigh, and calf) using skin temperature probes. Mean skin temperature and mean body temperature were calculated to assess changes in peripheral and overall thermal status. | 5-minutes before and 5-minutes after the 30-minute experimental/control condition. |
| Thermal sensation and shivering responses | Thermal sensation was assessed using the American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE) 7-point Thermal Sensation Scale, ranging from -3 (cold) to +3 (hot), where 0 indicates thermal neutrality. Shivering responses were assessed using the Bedside Shivering Assessment Scale (BSAS) ranging from 0 (no shivering) to 3 (severe shivering), with higher scores indicating greater shivering severity. | During the 30-minute experimental/control condition at 10-minute intervals. |
| Grigg MJ, Thake CD, Allgrove JE, Broom DR. Effects of cold-water immersion on energy expenditure, ad-libitum energy intake and appetite in healthy adults. Physiol Behav. 2025 Jul 1;296:114914. doi: 10.1016/j.physbeh.2025.114914. Epub 2025 Apr 10. |
| ID | Term |
|---|---|
| D005518 | Food Preferences |
| ID | Term |
|---|---|
| D005247 | Feeding Behavior |
| D001519 | Behavior |
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