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The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sulforaphane Supplementation Only | Experimental | In this intervention, participants will consume commercially available broccoli sprout extract capsules daily for 4 days (20 mg/day) . Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period. |
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| Exercise Only | Active Comparator | In this intervention, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period. |
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| Sulforaphane Supplementation and Exercise | Experimental | In this intervention, participants will consume commercially available broccoli sprout extract tablets daily for 4 days. In addition, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sulforaphane-rich Broccoli Sprout Extract | Dietary Supplement | Broc Elite Plus stabilized sulforaphane capsules from Mara Labs |
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| Measure | Description | Time Frame |
|---|---|---|
| Change in peripheral blood mononuclear cell oxygen consumption rate | OCR will be measured using a Seahorse Analyzer before and after each 4-day intervention | 0-4 days |
| Measure | Description | Time Frame |
|---|---|---|
| TNF-alpha secretion from LPS-stimulated whole blood | TNF-alpha secretion from LPS-stimulated whole blood will be measured via ELISA before and after each 4-day period | 0-4 days |
| Change in % of classical monocytes before and after the 6-week intervention |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Hashim Islam Principle Investigator, Ph.D | Contact | 250-807-9122 | hashim.islam@ubc.ca |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Reichwald Health Sciences Centre | Recruiting | Kelowna | British Columbia | V1V 1V7 | Canada |
IPD will not be shared to ensure the privacy and confidentiality of participants, in accordance with ethical guidelines and data protection regulations. Additionally, this was not included in the informed consent, which did not cover data sharing or address potential risks of re-identification.
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Randomized-crossover design
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| High Intensity Interval Treadmill Running | Other | 4 cycles of: 4 minutes of intense treadmill running (85-95% HR max) followed by 3 minutes of slow, easy running. |
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The % of classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention |
| 0-4 days |
| Change in % of intermediate monocytes before and after the 4-day intervention | The % of intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention | 0-4 days |
| Change in % of non-classical monocytes before and after the 4-day intervention | The % of non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention | 0-4 days |
| Change in TLR-4 expression on classical monocytes before and after the 4-day intervention | TLR-4 expression on classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention. | 0-4 days |
| Change in TLR-4 expression on intermediate monocytes before and after the 4-day intervention | TLR-4 expression on intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention. | 0-4 days |
| Change in TLR-4 expression on non-classical monocytes before and after the 4-day intervention | TLR-4 expression on non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention | 0-4 days |
| Change in white blood cell counts before and after the 4-day intervention | White blood cell counts will be measured using a hematology analyzer at baseline and after the 4-day intervention | 0-4 days |
| TNF-alpha secretion from PolyIC-stimulated whole blood | TNF-alpha secretion from PolyIC-stimulated whole blood will be measured via ELISA before and after each 4-day period | 0-4 days |
| Change in peripheral blood mononuclear cell extracellular acidification rate before and after the 4-day period | PBMC ECAR will be measured using a Seahorse Analyzer at baseline and after the 4-day intervention | 0-4 days |
| Change in PBMC mitotracker green staining before and after the 4-day period | PBMC mitotracker green will be measured using flow cytometry before and after the 4-day intervention period | 0-4 days |
| Change in PBMC mitoSOX red staining before and after the 4-day period | PBMC mitoSOX red will be measured using flow cytometry before and after the 4-day intervention period | 0-4 days |
| Change in PBMC mitochondrial respiration before and after the 4-day intervention period | PBMC mitochondrial respiration will be measured using high-resolution respirometry before and after the 4-day period | 0-4 days |
| ID | Term |
|---|---|
| D007249 | Inflammation |
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D001519 | Behavior |
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