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| ID | Type | Description | Link |
|---|---|---|---|
| UAQ-14172 | Other Identifier | Universidad Autónoma de Querétaro |
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| Name | Class |
|---|---|
| Universidad Autónoma de Querétaro | OTHER |
| National Council of Science and Technology, Mexico | OTHER |
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This study aims to evaluate the impact of a community-based nutritional intervention based on the Planetary Health Diet on dietary patterns, gut microbiota composition, and health indicators in adult women living in rural communities in Querétaro, Mexico.
Participants will receive nutritional education, individualized counseling, and practical workshops focused on improving dietary habits and promoting sustainable food choices. The intervention is designed using behavioral and community-based approaches to facilitate long-term adherence to healthy and environmentally sustainable diets.
Outcomes will be assessed before and after the intervention, including changes in diet quality, anthropometric measures, gut microbiota composition, and environmental impact indicators such as carbon footprint, water use, and land use.
This study is a community-based, quasi-experimental intervention designed to evaluate the effects of a nutritional program based on the Planetary Health Diet (PHD) on dietary intake, gut microbiota composition, anthropometric outcomes, and environmental sustainability indicators in adult women from rural communities in Querétaro, Mexico.
The intervention is developed using the Intervention Mapping framework and is grounded in Social Cognitive Theory, addressing behavioral, environmental, and psychosocial determinants of dietary practices. The program includes educational sessions, individualized nutritional counseling, and practical workshops focused on sustainable food preparation and dietary behavior change.
Participants are women aged 18 to 59 years residing in rural communities who meet inclusion criteria and provide informed consent. The intervention will be implemented over a defined period, with baseline and post-intervention assessments.
Primary outcomes include adherence to the Planetary Health Diet, measured using the EAT-Lancet Diet Index (ELD-I). Secondary outcomes include changes in gut microbiota composition assessed through 16S rRNA sequencing, anthropometric indicators (weight, BMI, waist circumference), and environmental impact metrics such as carbon footprint, water use, and land use.
Microbiota analysis will be conducted using fecal samples, with DNA extraction, amplification of the V4 region of the 16S rRNA gene, sequencing on an Illumina MiSeq platform, and analysis using QIIME software.
Statistical analysis will include descriptive and inferential methods, including paired comparisons, ANCOVA, regression models, and multivariate analyses to assess changes over time and associations between variables.
The study follows ethical principles outlined in the Declaration of Helsinki and has been approved by the Bioethics Committee of the Universidad Autónoma de Querétaro.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Planetary Health Diet Intervention | Experimental | Participants receive a community-based nutritional intervention aimed at improving adherence to the Planetary Health Diet. The intervention includes educational sessions, individualized counseling, and practical workshops focused on sustainable dietary practices and behavior change. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Community-Based Planetary Health Diet Intervention | Behavioral | This behavioral intervention includes educational sessions, individualized nutritional counseling, and practical workshops designed to promote adherence to the Planetary Health Diet. The intervention focuses on improving dietary patterns and encouraging sustainable food choices using community-based strategies and behavior change techniques. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in adherence to the Planetary Health Diet | Adherence to the Planetary Health Diet will be assessed using the EAT-Lancet Diet Index (ELD-I), which evaluates compliance with recommended intake ranges for key food groups and provides a composite score reflecting diet quality and sustainability. Scores range from 0 to 14, with higher scores indicating greater adherence to the Planetary Health Diet and a more sustainable dietary pattern. | Baseline to post-intervention (approximately 12 weeks) |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in gut microbiota composition | Gut microbiota composition will be assessed using 16S rRNA gene sequencing from fecal samples. Analysis will include alpha diversity (e.g., Shannon index), beta diversity (e.g., Bray-Curtis dissimilarity), and relative abundance of bacterial taxa. | Baseline to post-intervention (approximately 12 weeks) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Olga P García, PhD | Universidad Autonoma de Querétaro | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universidad Autónoma de Querétaro | Querétaro City | Querétaro | 76230 | Mexico |
Individual participant data (IPD) will not be shared due to ethical and privacy considerations. The study involves participants from rural communities, and sharing individual-level data may pose a risk of identification despite anonymization. Additionally, consent obtained from participants does not include provisions for public data sharing.
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This is a single-group, quasi-experimental study in which all participants receive a community-based nutritional intervention based on the Planetary Health Diet. Outcomes are assessed before and after the intervention to evaluate changes over time.
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| Change in dietary carbon footprint |
Diet-related carbon footprint will be estimated using environmental indicator data for the Mexican diet and dietary intake information obtained from a semi-food frequency questionnaire (SFFQ). |
| Baseline to post-intervention (approximately 12 weeks) |
| Change in dietary water footprint | Diet-related water footprint will be estimated using environmental indicator data for the Mexican diet and dietary intake information obtained from a semi-food frequency questionnaire (SFFQ). | Baseline to post-intervention (approximately 12 weeks) |
| Change in dietary land use | Diet-related land use will be estimated using environmental indicator data for the Mexican diet and dietary intake information obtained from a semi-food frequency questionnaire (SFFQ). | Baseline to post-intervention (approximately 12 weeks) |
| Change in total energy intake | Total dietary energy intake will be assessed using 24-hour dietary recalls and expressed in kilocalories per day. | Baseline to post-intervention (approximately 12 weeks) |
| Change in body weight | Body weight will be measured in kilograms using a calibrated digital scale. | Baseline to post-intervention (approximately 12 weeks) |
| Change in body mass index | Body mass index (BMI) will be calculated as weight in kilograms divided by height in meters squared (kg/m²). | Baseline to post-intervention (approximately 12 weeks) |
| Change in waist circumference | Waist circumference will be measured in centimeters using standardized anthropometric procedures. | Baseline to post-intervention (approximately 12 weeks) |