This study aims to investigate the relationship between NLRP3 inflammasome activation and different stages of periodontitis, with a focus on its association with inflammaging. Periodontitis is a chronic inflammatory disease affecting the supporting tissues of the teeth, and aging-related inflammation may influence its progression.

The study will include participants with different stages of periodontitis as well as healthy individuals. Clinical periodontal parameters will be recorded, and biological samples such as saliva or gingival crevicular fluid will be collected to measure levels of NLRP3 inflammasome components and inflammatory markers.

The results of this study may help improve understanding of the role of inflammation and aging in periodontal disease and could contribute to better diagnosis and treatment strategies.

Periodontitis is a chronic inflammatory disease characterized by progressive destruction of the supporting structures of the teeth. Recent evidence suggests that the NLRP3 inflammasome plays a significant role in the pathogenesis of periodontal disease by regulating the production of pro-inflammatory cytokines such as interleukin-1β.

In addition, inflammaging, defined as low-grade chronic inflammation associated with aging, may contribute to increased susceptibility and severity of periodontal disease. However, the relationship between NLRP3 inflammasome activation and different stages of periodontitis, particularly in the context of inflammaging, is not fully understood.

This observational study aims to evaluate the expression levels of NLRP3 inflammasome components in subjects with varying stages of periodontitis compared to healthy controls. Clinical periodontal parameters, including probing depth, clinical attachment level, and bleeding on probing, will be recorded. Biological samples such as saliva and/or gingival crevicular fluid will be collected for laboratory analysis.

The primary objective is to assess the association between NLRP3 activation and disease severity. Secondary objectives include evaluating the correlation between inflammasome markers and aging-related inflammatory changes.

The findings of this study may provide insight into the molecular mechanisms underlying periodontitis progression and highlight potential biomarkers for early diagnosis and therapeutic targets.

Inclusion Criteria:

• 1. Male and Female 2. Aged from18 years and above 50. 3. Have at least 15 natural teeth excluding wisdoms. 4. Provide informed consent and willingness to cooperate with study protocol. 5. Stage I periodontitis and Grade A

  • CAL of 1-2 mm
  • Probing depths typically ≤ 3 6. Periodontitis stage II or stage III and Grads B or C patients:
  • CAL from 3-4 mm.
  • Maximum probing depth ≤ 5 mm, mostly horizontal bone loss

Exclusion Criteria:

• 1- History of antibiotic in previous three months. 2- Pregnant or lactating female. 3- Treatment with any systemic drug. 4- History of systemic disease. 5- Patients who received periodontal treatment in last 6 months

he study population will include adult participants aged 18 years and above attending the dental clinic. Participants will be categorized into healthy controls and patients with different stages of periodontitis based on clinical periodontal examination. Individuals will be systemically healthy and not under medications affecting periodontal status. Both males and females will be included. Clinical parameters such as probing depth, clinical attachment level, and bleeding on probing will be recorded, and biological samples (saliva and/or gingival crevicular fluid) will be collected for analysis of NLRP3 inflammasome-related biomarkers.

Individual participant data will not be shared due to ethical considerations and to protect participant confidentiality, as this is an academic observational study without a formal data-sharing framework.