Identifying Terpenoid- and Carotenoid-modifying Enzymes in the Human Gut

Phage-Enabled Mining of Gut Metagenomes for Terpenoid and Carotenoid Metabolism

In this study, the investigators will collect fecal samples from healthy adults. The investigators will extract DNA from these fecal samples and use microbiological techniques to determine whether this DNA encodes enzymes that can react with certain chemicals called terpenoids and carotenoids. The investigators will additionally collect dietary information via a questionnaire.

Inclusion Criteria:

• Body Mass index <25

Exclusion Criteria:

• Pregnant
• Taken antibiotics or antifungals in the past 3 months
• Taken probiotic supplements in the past 3 months
• Diarrhea in the past month