Not provided
Not provided
| ID | Type | Description | Link |
|---|---|---|---|
| R21AT012805 | U.S. NIH Grant/Contract | View source |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| National Center for Complementary and Integrative Health (NCCIH) | NIH |
Not provided
Not provided
Not provided
Not provided
In this study, the investigators will collect fecal samples from healthy adults. The investigators will extract DNA from these fecal samples and use microbiological techniques to determine whether this DNA encodes enzymes that can react with certain chemicals called terpenoids and carotenoids. The investigators will additionally collect dietary information via a questionnaire.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy adults |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| No Intervention: Observational Cohort | Other | No intervention |
|
| Measure | Description | Time Frame |
|---|---|---|
| Number of unique gut metagenomic enzymes with carotenoid-modifying activities for each participant. | Subjects' fecal metagenomic DNA will be extracted from their fecal sample, and random fragments of this metagenomic DNA will be inserted into an E. coli expression vector. This vector library will be transformed into an E. coli strain that will change color if a carotenoid-modifying gene is present. We expect to obtain about one million E. coli clones per participant. These clones will be spread on agar plates, and the colonies exhibiting different colors will be recovered and sequenced. The sequences of each participant will be collected and annotated to determine putative carotenoid-modifying enzymes, and the number of unique enzymes will be saved. | Day 1 |
Not provided
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Healthy adults
Not provided
Not provided
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| NC State University | Raleigh | North Carolina | 27606 | United States |
Fecal samples and data derived from them, including metagenome, metatranscriptome, metaproteome, and metabolome data will be provided to other researchers provided that they agree not to re-identify participants.
Not provided
Not provided
Not provided
Not provided
Not provided
| Type | Includes Protocol | Includes SAP | Includes ICF | Document Label | Document Date | Document Uploaded Date | Document File Name |
|---|---|---|---|---|---|---|---|
| Prot | Yes | No | No | Study Protocol | Jul 22, 2025 | May 9, 2026 | Prot_000.pdf |
| ICF | No | No | Yes | Informed Consent Form | Jul 22, 2025 | May 9, 2026 | ICF_001.pdf |
Not provided
Not provided
Not provided
Not provided
Feces