This is a randomized, placebo-controlled trial with a cross-over component. It investigates the effect of non-invasive acupoint stimulation on stress in young and middle-aged women. A total of 90 participants will be enrolled: 60 with a Liver-Qi Stagnation constitution and high perceived stress, and 30 without this constitution as a non-intervention baseline control. The 60 participants with the target constitution will be randomized into two groups. One group (n=30) will undergo a cross-over intervention, sequentially receiving steam-warm acupressure and simple acupressure (at five acupoints) in a randomized order, separated by a washout period. The other group (n=30) will receive placebo stimulation. The primary outcome is the change in the Perceived Stress Scale (PSS-14) score. Secondary outcomes include physiological stress responses.

Inclusion Criteria:

• Female, aged 20-40 years.

• Regular daily routine with relatively fixed wake-up and bedtimes, sufficient sleep (7-9 hours), regular diet, moderate physical activity, and avoidance of shift work.

• Intact structure of major organ systems, with no active diseases or pathological damage.

• Regular menstrual cycle (21-35 days) and normal menstrual period (3-7 days).

• For the experimental group (Qi Yu constitution group):

  • Perceived Stress Scale (PSS-14) score ≥ 43.

  • Qi Yu Constitution Subscale score ≥ 40.

  • Diagnosis by a qualified TCM physician according to standard procedures, confirming characteristics of Qi Yu constitution, including (physical symptoms partially required):

    • Body type: Tending to be thin.
    • Psychological characteristics: Introverted, unstable, sensitive, suspicious, with significant emotional fluctuations.
    • Somatic symptoms: Fullness or migrating pain in the chest and hypochondrium, frequent sighing; sensation of a foreign body in the throat (globus hystericus); sleep disturbances (difficulty falling asleep, early awakening). Poor appetite, frequent belching.
    • Tongue and pulse: Pale red tongue with thin white coating, wiry and thin pulse.

• For the control group (non-Qi Yu constitution group):

  • Perceived Stress Scale (PSS-14) score ≤ 42.
  • Qi Yu Constitution Subscale score < 30.
  • No relevant clinical manifestations upon TCM examination.

• Signed informed consent form, voluntarily participating in the trial.

• Willing to comply with all testing requirements.

• No infection or bleeding tendency on the skin of the five acupoint areas (GV20/Baihui, LI4/Hegu, LR3/Taichong, PC6/Neiguan, SP6/Sanyinjiao), not affecting the use of the study products.

Exclusion Criteria:

• Participation in any other clinical trial evaluation within the past 1 month.
• Presence of organic diseases, such as various malignant tumors, certain chronic diseases (heart disease, diabetes, anemia, etc.), certain gynecological diseases (uterine fibroids, endometriosis, severe mammary hyperplasia, etc.), or certain endocrine diseases (hyperthyroidism or hypothyroidism, etc.).
• Individuals with hypothalamic-pituitary-adrenal (HPA) axis dysfunction, such as those with endocrine diseases (e.g., hyper/hypothyroidism, Cushing's syndrome, congenital adrenal hyperplasia, primary chronic adrenocortical insufficiency).
• Individuals diagnosed with major depressive disorder or anxiety disorder, bipolar disorder, schizophrenia, etc.
• Use of antidepressants, anti-anxiety medications, glucocorticoids, or immunosuppressants within the past 4 weeks.
• Recent (within the past 3 months) suicidal ideation or self-harm behavior.
• Receipt of acupuncture, massage, or other TCM treatments or psychological interventions (e.g., cognitive behavioral therapy) within the past 4 weeks.
• Suffering from autoimmune diseases requiring long-term use of hormones.
• Alcohol or drug dependence.
• Experienced major stressful life events (e.g., bereavement of a close relative suffering a major psychological blow) in the past year.
• Physically weak or unable to tolerate the arithmetic stress in the MIST task.
• Conditions such as epilepsy, hypertension, or heart disease that are unsuitable for undergoing induced psychological stress.
• Inability to cooperate in completing the MIST task (e.g., due to visual/auditory impairment, cognitive dysfunction).
• Inability or unwillingness to attend all study visits and comply with the treatment regimen.