Due to the current lack of evidence regarding the influence of fluid intake on the amount of leukocyte- and platelet-rich fibrin fraction present in blood samples, this study aims to investigate the positive influence of this factor on the body's own matrix.

Platelet-rich fibrin, a fibrin-rich autologous matrix obtained from autologous blood via centrifugation, contains not only fibrin but also important growth factors and a large number of white blood cells, all of which make a significant contribution to tissue regeneration. The obtained fibrin matrix can be used as a membrane, as a clot (A-PRF), or as a liquid (i-PRF) to positively influence the healing process.

The novelty of this work lies in shifting the focus from purely technical centrifugation parameters to a patient-centered, easily modifiable biological variable. Hydration status represents a low-cost, non-invasive, and immediately applicable strategy to optimize autologous blood concentrates. These findings are directly relevant to clinicians seeking to enhance the predictability and efficiency of PRF-based regenerative procedures in implant dentistry.

Inclusion Criteria:

- Healthy adults

Exclusion Criteria:

• coagulation disorders
• anticoagulant medication
• pregnancy
• acute infections
• systemic diseases with the potential to affect wound healing or blood composition

Data will be shared upon reasonable request. Data sheets containing anonymized measurements, sorted by outcome variable, can be shared. To request this, please contact us via email and explain your reason for wanting the data. The data will be shared personally; no public link will be provided.