Phenotypic and Genotypic Detection of Colistin Resistance

Phenotypic and Genotypic Detection of Colistin Resistance Among Multi Drug Resistant Gram-negative Bacteria in Assiut University Hospital

• Evaluation of the disk elution test as reliable routine method for detection of colistin resistant among multidrug resistant gram-negative bacteria compared with broth microdilution.
• Molecular detection of colistin resistant in multidrug-resistant gram-negative bacteria.

Inclusion Criteria:

-Multidrug-resistant gram-negative isolates will be collected from clinical specimens (blood, urine, sputum, wound swabs, etc.) obtained from hospitalized patients in Assiut University Hospitals.

Exclusion Criteria:

• gram-positive bacteria
• carbapenem-sensitive gram-negative bacteria
• repeated sample for same patient or different samples from the same patient