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| ID | Type | Description | Link |
|---|---|---|---|
| Beasiswa Pendidikan Indonesia | Other Identifier | Center for Higher Education Funding and Assessment, under The Ministry of Higher Education, Science and Technology | |
| FK-KMK | Other Identifier | Gadjah Mada University |
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The aim of this research is to analyze the effect of a Lactiplantibacillus plantarum Dad-13 supplementation on the gut microbiota profile and performance of adolescent athletes.
An athlete's physical performance can be influenced by the balance of gut microbiota in the digestive tract. Research shows that as many as 96% of ultramarathon participants (161 km) experience several digestive symptoms such as belching, nausea, vomiting and stress with a failure to reach 35.6% in completing the race. The diversity of an athlete's gut microbiota is higher than that of a normal person. This study is a true experimental study with a double-blind, pre- and posttest randomized controlled trial with a total of 30 athletes as respondents. There are two treatments: the first group will be given 1 gram of capsule preparation containing the probiotic Lactiplantibacillus plantarum Dad-13 given twice/day and the control group given a placebo capsule twice/day. The probiotic intervention was given at breakfast and dinner for 8 weeks. Measurement of the microbiota profile comes from fecal samples to further measure digestive tract health, SCFA levels, and calprotectin concentrations. Analysis of alpha diversity and beta diversity using Microbiome Analyst software. Physical performance was measured using VO2max using a direct laboratory method, namely the COSMED K5 instrument, before and after the intervention in kg/mL. Food intake was measured using a 24-hour food recall method and physical activity using an activity recall method.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control group | Experimental | placebo capsule twice/day |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Probiotic Lactiplantibacillus plantarum Dad-13 | Dietary Supplement | Lactiplantibacillus plantarum Dad-13 is an indigenous probiotic strain isolated from the curd produced by spontaneous fermentation of buffalo milk in bamboo containers and originates from Minangkabau, West Sumatra, Indonesia. |
| Measure | Description | Time Frame |
|---|---|---|
| gut microbiota profile | Gut microbiota profiles were measured from fecal samples, then isolated using 16S rDNA sequencing. Fecal samples were collected in the morning. Results: alfa and beta diversity | From pre treatment and the end of treatment at 8 weeks |
| Short chain fatty acid profile | The total level or amount of short chain fatty acid metabolites produced from the fermentation process in the intestine obtained from feces. Results: Total amount of SCFA, acetic acid, propionic acid, butyric acid | pre treatment and end of treatment at 8 weeks |
| Physical Performance | The physical performance measurement used was VO2max. The VO2max measurement method used a direct laboratory method with COSMED K5 before and after the intervention, with units of kg/mL. | pre treatment and the end of treatment at 8 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| Fecal calprotectin concentration | Protein in white blood cells that acts as an inflammation marker with measurement using ELISA. Fecall samples was used. | pre treatment and the end of treatment at 8 weeks |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Natalia Desy Putriningtyas, Dietisien., M.Gizi | Contact | +6285640885101 | nataliadesyputriningtyas@mail.ugm.ac.id | |
| Lilly Arsanti Lestari, Dr. | Contact | +62 877-3849-5045 |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Balai Pembinaan dan Pelatihan Olahraga Pelajar Provinsi Jawa Tengah | Semarang | Central Java | 50231 | Indonesia |
IPD that underlie results in a publication.
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