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The present study has the purpose to develop an innovative method for obtaining HSCs with a high yield. HSCs extracted from UCB using the traditional method followed by several steps of centrifugation will be compared to the new method which exploit a new biocompatible liquid, capable to separate the HSCs without causing mechanical stress to cells. The potential of ozone treatment to stimulate cells proliferation will be also assessed. Since ozone has been identified as an adjuvant to sustain cytoprotective pathways increasing cell performance, HSCs obtained from UCB samples will be treated by flowing ozone at low concentrations (between 2 and 20 μg/mL) in a mixture 1:1 with oxygen.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Umbilical cord blood collection | Other | the study purpose is to collect between 70 to 200 ml of blood for each donor from both caesarean and natural births, post-partum. The umbilical cord will be clamped, as directed by the surgeon performing the procedure, in two places: close to the placenta and close to the baby. |
| Measure | Description | Time Frame |
|---|---|---|
| Efficacy of HSCs extraction | Evaluation of the efficacy of HSCs extraction with a new method which exploit a new biocompatible liquid, capable to separate the HSCs without causing mechanical stress to cells. | up to 24 months |
| Measure | Description | Time Frame |
|---|---|---|
| Effect of ozone treatment on the viability/yield of HSCs. | The potential of ozone treatment to stimulate cells proliferation will be assessed | up to 24 months |
| cells recovery after freezing and thawing |
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Inclusion Criteria:
Exclusion Criteria:
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Healthy women who have signed the informed consent for umbilical cord donation for research purposes.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Arsenio Spinillo, MD | Contact | 0382503792 | a.spinillo@smatteo.pv.it |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Fondazione IRCCS Policlinico San Matteo di Pavia | Recruiting | Pavia | Lombardy | 27100 | Italy |
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Umbilical cord blood
Vitality (number of cells) by trypan blue staining and cell counting.
| up to 24 months |