This randomized clinical study evaluates the antibacterial effectiveness and postoperative pain following root canal irrigation using the Triton irrigation protocol in necrotic mandibular molars. Thirty-six patients requiring root canal treatment will be included. The study aims to compare bacterial reduction and postoperative pain outcomes after treatment. Results may help improve irrigation protocols and patient comfort during endodontic therapy.

Root canal disinfection is a critical factor affecting the success of endodontic treatment, especially in teeth with necrotic pulps. Irrigation protocols play an essential role in reducing bacterial load inside the root canal system. The Triton irrigation protocol has been introduced to enhance antimicrobial effectiveness and improve debris removal.

This randomized clinical trial will include 36 patients diagnosed with necrotic mandibular molars indicated for root canal treatment. Participants will receive treatment following the assigned irrigation protocol. Bacterial samples will be collected before and after irrigation to evaluate antibacterial effectiveness. Postoperative pain will be assessed using a standardized pain scale during the follow-up period.

The findings of this study are expected to provide clinical evidence regarding the effectiveness of the Triton irrigation protocol in improving disinfection outcomes and reducing postoperative discomfort

Inclusion Criteria:

• Patients aged 18-45 years with no sex predilection
• Medically free Patients with no systemic conditions that can affect post operative pain response.
• Patients should not be taking any pain medications 24 hours before intervention
• Positive patients' acceptance for participating in the study
• Patient able to sign informed consent
• Permanent mandibular molars diagnosis with pulpal necrosis with asymptomatic apical periodontitis.
• Mandibular molars without any anatomical variations or severe curvatures with mesial roots configuration weine class 3 and a distal root with 2 distal canals

Exclusion Criteria:

• Medically compromised patients

  • Patients who have received antibiotic therapy within the last 2 weeks.
  • Pregnant or lactating women.
  • Patients with known allergy to any of the irrigants used.
  • Patients with swelling or acute peri-apical abscess or fistulous tract.
  • Teeth that have:
  • Wide or open apex .
  • Vital pulp tissue .

Individual participant data will not be shared to protect participant confidentiality and privacy.

Participants and outcome assessors were blinded to group allocation, while the operator delivering the intervention was aware of the assigned treatment.