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| ID | Type | Description | Link |
|---|---|---|---|
| 25PRE1410388 | Other Grant/Funding Number | American Heart Association | |
| A176000 | Other Identifier | UW Madison | |
| Protocol Version 2/21/26 | Other Identifier | UW Madison |
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The purpose of this study is to determine 1) if young adults with metabolic syndrome display endothelial dysfunction of the internal carotid artery in a sex-specific fashion and 2) if this endothelial dysfunction can be acutely improved with a single bout of exercise. This will be achieved by testing internal carotid artery function before and after a 30-minute bout of moderate intensity exercise. 48 young adults with and without metabolic syndrome will be enrolled and will be on study for approximately 5.5 hours over 4 study visits.
The goal of this project is to determine if young MetSyn patients exhibit carotid endothelial dysfunction (ED) in a sex-specific manner and if exercise can acutely restore internal carotid artery (ICA) function.
The investigators will test this by first quantifying ICA endothelial function using an inhaled gas stressor (CO2) to acutely increase ICA shear rate (SR) and measuring the vascular responsiveness to this increased SR. Carotid endothelial function has never been tested in this way in patients with MetSyn before. Then, a 30 min bout of cycling exercise will be performed followed by the same gas stressor test to determine if ICA endothelial function can be acutely improved in MetSyn.
The aims and hypotheses are to:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Young Male adults with MetSyn |
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| Young Female adults with MetSyn |
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| Young Male Healthy Controls |
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| Young Female Healthy Controls |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| exercise | Behavioral | 30 minute bout of cycling |
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| Measure | Description | Time Frame |
|---|---|---|
| Aim 1: Baseline ICA cSMD measures assessed by MetSyn versus healthy controls | Internal carotid artery cerebral shear-mediated dilation (cSMD) is measured by doppler ultrasound and reported in percentage increase in diameter. A resting baseline period for 5 minutes breathing room air followed by 30 seconds of breathing CO2-mixed air at +9 mmHg PETCO2 used to evoke an increase in blood flow to the ICA. | baseline |
| Aim 2: Baseline ICA cSMD measures assessed by MetSyn versus sex | Internal carotid artery cerebral shear-mediated dilation is measured by doppler ultrasound and reported in percentage increase in diameter. A resting baseline period for 5 minutes breathing room air followed by 30 seconds of breathing CO2-mixed air at +9 mmHg PETCO2 used to evoke an increase in blood flow to the ICA. | baseline |
| Aim 3: Post-exercise cSMD response by MetSyn versus healthy controls | Internal carotid artery cerebral shear-mediated dilation is measured by doppler ultrasound and reported in percentage increase in diameter. 30 minutes of exercise on a cycle ergometer will be performed at 80% of the ventilatory threshold determined during the graded exercise test. | measured during exercise and 10 and 60 minutes post-exercise |
| Measure | Description | Time Frame |
|---|---|---|
| Aim 3: Duration of Response to Exercise | Duration of post-exercise improvements will be investigated by testing ICA cSMD 10 min and 60 min post-exercise. 30 minutes of exercise on a cycle ergometer will be performed at 80% of the ventilatory threshold determined during the graded exercise test. For the duration of the exercise, indirect calorimetry will be used to assess VO2 to confirm the target workload of 80% of the ventilatory threshold. During this 30 minutes of exercise, ICA diameter and velocity will be measured using ultrasonography every 5 minutes for a 2-minute period. |
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Inclusion Criteria (MetSyn cohort):
Inclusion Criteria (Healthy cohort):
- meet age criteria
Exclusion Criteria:
Current smoker, defined as more than 5 cigarettes over past 30 days
Current diagnosis or history of:
Type II diabetes
Currently pregnant or breastfeeding
Current musculoskeletal injury Medication use known to influence cardiovascular function, other than oral contraceptives
Hormonal contraceptive use, such as intrauterine device or hormonal implant use
Participants with ICAs unsuitable for imaging, as assessed during the screening visit via doppler ultrasonography
Exclusion Criteria (healthy controls only):
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Young adult male and female participants with metabolic syndrome and age matched healthy male and female participants.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Shawn Bolin, MS | Contact | 608-263-6308 | sbolin@wisc.edu | |
| Logan Heenan, BS | Contact | 608-263-6308 | lheenan@wisc.edu |
| Name | Affiliation | Role |
|---|---|---|
| William Schrage, PhD | University of Wisconsin, Madison | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Wisconsin | Recruiting | Madison | Wisconsin | 53792 | United States |
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| ID | Term |
|---|---|
| D024821 | Metabolic Syndrome |
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D007333 | Insulin Resistance |
| D006946 | Hyperinsulinism |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| D000092025 | Rapid Diagnostic Tests |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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| Diagnostics | Diagnostic Test |
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| Inhaled CO2-mixed air | Other | Three gases of different composition are used to ensure that the proper PETCO2 administration desired for this study occurs in tandem with keeping inspired O2 content constant. This is achieved by used a 5% O2, 95% CO2 tank, a 5% O2, 95% N2 tank, and a 100% O2 tank. In tandem, the use of these three tanks allows for precise control of PETCO2 without a change in inspired O2. |
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| measured during exercise and 10 and 60 minutes post-exercise |
| D009750 |
| Nutritional and Metabolic Diseases |
| D001519 | Behavior |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D008919 | Investigative Techniques |
| D000067716 | Point-of-Care Testing |
| D019095 | Point-of-Care Systems |
| D010346 | Patient Care Management |
| D006298 | Health Services Administration |