This study aims to compare of effect of chlorhexidine gluconate and alcohol hand rubbing on healthcare-associated infection (HCAI) in neonatal intensive care unit at Alexandria University Children Hospital.

Inclusion Criteria:

Neonates <37 weeks gestational age admitted to NICU of Alexandria University Children's Hospitals

Exclusion Criteria:

• Patients with any of the following will be excluded:

  1. Obvious major congenital abnormalities, immunodeficiency and inborn error of metabolism.
  2. Early onset sepsis (before the third day).
  3. Infants more than 72 hours of age at the time of randomization.
  4. Parental consent lacking/refusal.