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Blood culture (BC) is the gold standard for the definitive diagnosis of bacteraemia, providing the opportunity to recover and identify the causative organism, and optimize subsequent antimicrobial treatment. Detection of bacteraemia is critical for the management of sepsis. This study aims to examine the performance of the blood culture pathway in Gloucestershire Hospitals Trust using data analysis, questionnaires and focus groups. . Information collected will be used to design interventions that could be used to optimise the blood culture pathway
Retrospective data collection relating to the blood volume collected and time to incubation of adult blood culture sets (adult sets comprises of both aerobic and anaerobic blood culture bottle) received in the microbiology laboratory, between February 2023 and April 2024. These samples are routinely collected as standard of care and not for the purpose of this research.
Staff members that collect adult blood cultures within the Trust (nurses, Physician's associates and doctors) will be invited to volunteer for the study. Staff members will participate by completing the questionnaire and/or attending focus groups.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Staff members that collect adult blood cultures within the Trust |
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| Measure | Description | Time Frame |
|---|---|---|
| Compliance to optimal blood culture (BC) vol (8-10 mls per bottle). | Volume of blood cultures measured in ml. | Retrospective data collection from period February 2023 to April 2024. |
| Compliance to 4- hour time target to incubation of blood culture from collection. | Time taken from collection of blood culture to incubation in the laboratory, recorded in hours and minutes | Retrospective data collection from period February 2023 to April 2024 |
| Time from collection of blood cultures to targeted antibiotics, hours and minutes | Measurement of time (hours, minutes) from time of blood culture collection to time of targeted antibiotic prescribing | Retrospective data collection from period February 2023 to April 2024. |
| Staff experiences related to taking blood cultures | Staff experiences using questionnaires and focus group discussions exploring facilitators and barriers relating to blood culture collection . Staff members will participate by completing the questionnaire and/or attending focus groups. | Approximately 10 minutes to complete electronic questionnaire. Attendance at single FOCUS group at agreed time. |
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Inclusion Criteria:
Secondary data:
- adult blood culture sets received in the microbiology laboratory, between February 2023 and April 2024.
Staff member participants
- Staff members that collect adult blood cultures within the Trust
Exclusion Criteria:
Secondary data:
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Secondary data: adult blood culture sets received in the microbiology laboratory, between February 2023 and April 2024.
Staff member participants: Staff members that collect adult blood cultures within the Trust
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Gloucestershire Royal Hospital | Recruiting | Gloucester | Gloucestershire | GL1 3NN | United Kingdom |
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