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This study investigates the effects of moderate physical exercise and cyclic sighing (breathing training) on stress, cognition, and physiological markers in young adults. It is a randomized cross-over trial with two interventions. Each participant will take part in two intervention sessions:
A one-week washout period between sessions is planned to minimise carry-over effects and to ensure participants' physiological state is similar before the next session. During this week, participants will wear Polar Ignite wrist-worn devices to monitor physical activity and sleep.
A total of 30 participants (you adults) will be enrolled.
Measurements performed before and after each intervention include:
Baseline questionnaires completed by participants the day before the study:
The study aims to understand how brief interventions like moderate exercise and breathing techniques affect stress, cognitive performance, and physiological responses in young adults.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Moderate-Intensity Physical Activity (PA) | Experimental | Participants perform 15 minutes of stationary cycling at moderate intensity. |
|
| Breathing Training - Cyclic Sighing (CS) | Experimental | Participants perform 15 minutes of cyclic sighing. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Moderate-Intensity Physical Activity (PA) | Behavioral | Participants perform 15 minutes of stationary cycling at moderate intensity. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Mean EEG spectral power in theta, alpha, low beta, high beta, gamma frequency bands during resting-state (eyes-open and eyes-closed) | Mean spectral power in theta, alpha, low beta, high beta, and gamma frequency bands derived from resting-state EEG recordings collected during eyes-open and eyes-closed conditions. Spectral power will be calculated separately for eyes-open and eyes-closed resting-state conditions. | Baseline: immediately before intervention; Post-intervention: immediately after completion of the intervention period |
| Heart Rate Variability (HRV) | HRV will be recorded at three time points. Baseline HRV will be calculated as the average over a 5-minute resting-state period immediately before intervention. HRV during the intervention will be calculated as the average over the entire intervention session (periprocedural). Post-intervention HRV will be calculated as the average over a 5-minute resting-state period immediately after intervention. | Baseline (average over 5-minute resting-state immediately before intervention); During intervention (average over entire session, periprocedural); Post-intervention (average over 5-minute resting-state immediately after intervention). |
| Measure | Description | Time Frame |
|---|---|---|
| Mean EEG spectral power in theta, alpha, low beta, high beta, and gamma frequency bands during N-back task | Mean spectral power in theta, alpha, low beta, high beta, and gamma frequency bands derived from EEG recordings collected during performance of the N-back cognitive task. Spectral power will be calculated separately for each frequency band and averaged across all EEG channels. | Baseline (during N-back task immediately before each intervention session, periprocedural); Post-intervention (during N-back task immediately after each intervention session, periprocedural) |
| Measure | Description | Time Frame |
|---|---|---|
| Psychological Well-Being measured with the Short Version of Ryff's Psychological Well-Being Scale | Participants complete the Short Version of Ryff's Psychological Well-Being Scale, a self-reported questionnaire assessing psychological well-being across multiple dimensions. Items are rated on a scale from 1 (strongly disagree) to 6 (strongly agree), giving a total possible score range of 18-108, where higher scores indicate higher levels of psychological well-being. |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Jóźwiak | Contact | (61) 835 53 49 | b.jozwiak@awf.poznan.pl |
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De-identified individual participant data (IPD) will be shared in the RepOD repository after study completion. An embargo of up to 36 months may apply to allow for publication of primary study results. Data will be available for scientific research purposes.
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| Breathing Training - Cyclic Sighing (CS) | Behavioral | Participants perform 15 minutes of cyclic sighing (a breathing technique). |
|
| Mean EEG spectral power in theta, alpha, low beta, high beta, and gamma frequency bands during intervention session | Mean spectral power in theta, alpha, low beta, high beta, and gamma frequency bands derived from EEG recordings collected during the intervention session. Spectral power will be calculated separately for each frequency band and averaged across all EEG channels. | During intervention session (periprocedural, averaged over entire session) |
| Perceived Stress measured with the Subjective Units of Distress Scale (SUDS) | Participants rate their perceived stress using the Subjective Units of Distress Scale (SUDS), a self-reported scale ranging from 0 (no stress) to 100 (extreme stress), where higher scores indicate higher perceived stress. | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Cognitive Performance (N-back) | Working memory assessed using N-back test with Emotiv app. | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Blood Pressure | Systolic and diastolic blood pressure measured using standard protocols. | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Pain Threshold | Pain threshold measured using a dolorimeter (algorimeter) | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Positive and Negative Affect measured with the Positive and Negative Affect Schedule - Short Form (PANAS-SF) | Participants complete the Positive and Negative Affect Schedule - Short Form (PANAS-SF), a self-reported scale assessing positive and negative affect. Each subscale contains 10 items rated from 1 (very slightly or not at all) to 5 (extremely), giving a possible score range of 10-50 for each subscale. Higher scores indicate higher levels of positive or negative affect, depending on the subscale. | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Heart Rate | Heart rate measured using standard protocols. | Baseline: immediately before each intervention session; Post-intervention: immediately after each intervention session |
| Day before first intervention. |
| Trait Mindfulness measured with the Mindful Attention Awareness Scale (MAAS) | Participants complete the Mindful Attention Awareness Scale (MAAS), a self-reported questionnaire measuring trait mindfulness. Items are rated on a scale from 1 (almost always) to 6 (almost never), with total scores ranging from 15 to 90. Higher scores indicate higher levels of trait mindfulness. | Day before first intervention. |
| Coping with Stress measured with the Brief COPE Inventory (mini-COPE) | Participants complete the Brief COPE Inventory (mini-COPE), a self-reported questionnaire assessing coping strategies and stress management. Each item is rated on a scale from 1 (I haven't been doing this at all) to 4 (I've been doing this a lot), with total subscale scores ranging from 2 to 8, depending on the subscale. Higher scores indicate greater use of the specific coping strategy. | Day before first intervention. |
| Chronotype measured with the Morningness-Eveningness Questionnaire (MEQ) | Participants complete the Morningness-Eveningness Questionnaire (MEQ) to assess chronotype, indicating morningness-eveningness preference. Items are scored to give a total score ranging from 16 to 86, where higher scores indicate greater morningness and lower scores indicate greater eveningness. | Day before first intervention. |
| Global Physical Activity measured with the Global Physical Activity Questionnaire (GPAQ) | Participants complete the Global Physical Activity Questionnaire (GPAQ) to assess baseline physical activity levels. Responses are used to calculate total physical activity expressed in MET-minutes per week, with higher scores indicating higher levels of physical activity. | Day before first session. |
| Sleep Quality and Duration (Pittsburgh Sleep Quality Index) | Participants complete the Pittsburgh Sleep Quality Index (PSQI) to assess sleep quality and average sleep duration. | Day before first session. |
| Objective Sleep and Physical Activity (Polar Ignite) | Participants wear Polar Ignite wrist-worn devices to monitor sleep quality and duration, as well as daily physical activity levels. | During the 1-week washout period between intervention sessions. |