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Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention.
Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups.
Bacterial identification by Gram stain, culture and biochemical rections
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Group 1 patients with klebsiella pneumoniae infection | Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections. |
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| Group 2 patients without klebsiella pneumoniae | Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| . - Genotypic detection of carbapenemase genes by conventional PCR. | Genetic | Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections - analysisThe resistance pattern of the isolates will be detected by disc diffusion method. - phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR. |
| Measure | Description | Time Frame |
|---|---|---|
| 1 ISOLATION OF KLEB. by culture on macConkey agar | 6 months from January to may | |
| Antibiotic sensitivity testing of different antibiotics according to clsi 25 | 6 months from January to may | |
| Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae | 6 months from January to may | |
| Detection of resistance gene in isolates using conventional pcr | 6 months from June to December | |
| Detection of some virulence genes using conventional pcr | 6 months from June to December |
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Inclusion Criteria: Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.-
Exclusion Criteria:
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All selected patients subjected to complete history All samples transported to medical microbiology department
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Walaa Ismael, Demonstrator | Contact | +20114 8736091 | walaa.marzouk@med.sohag.edu.eg |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Faculty of medicine Sohag University | Sohag | Egypt |
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