Safety and Efficacy of Metabolically Armed Tumor-Infiltrating Lymphocytes (Meta10-TIL) for the Treatment of Advanced Solid Tumors

Safety and Efficacy of Metabolically Armed Tumor-Infiltrating Lymphocytes (Meta10-TIL) for the Treatment of Advanced Solid Tumors

A Study of Metabolically Armed Tumor-Infiltrating Lymphocytes (Meta10-TIL) Therapy for Patients With Advanced Solid Tumors

This is an open-label study. This study is indicated for advanced solid tumors. The selections of dose levels and the number of subjects are based on clinical trials of similar products and the outcomes of our preliminary clinical studies.

1. Main research objectives:

   To evaluate the safety of metabolically armed tumor-infiltrating lymphocytes (TILs) in patients with advanced solid tumors.

2. Secondary research objectives:

(1)To evaluate the objective response rate (ORR), duration of response (DOR), progression-free survival (PFS), and disease control rate (DCR) of metabolically armed tumor-infiltrating lymphocytes (Meta10-TIL) with RECIST v1.1; (2)To evaluate the overall survival (OS); (3) Characterize the pharmacokinetic (PK) and pharmacodynamic (PD) profiles of Meta10-TILs.

Inclusion Criteria:

• The patient or his/her guardian voluntarily signed the informed consent；
• Age >18 years and ≤70 years, male or female;
• Patients with advanced solid tumors who have been confirmed by histopathology or cytology:

Patients with advanced solid tumors who have failed prior standard treatments (due to disease progression or intolerance to toxicity), currently have no standard treatment options, or are unable to tolerate the current standard treatment for other reasons;

• The subject has residual lesions suitable for surgical resection (≥1.5 cm) or biopsy (core needle biopsy specimens: ≥4 passes with 16G needle or ≥6 passes with 18G needle) to generate tumor-infiltrating lymphocytes (TILs). For cervical cancer subjects, tumor tissue meeting either ≥0.5 cm in diameter or ≥400 mm³ in volume is acceptable. Fresh tumor tissue for TIL production should preferably be obtained from proximal metastatic lymph nodes or the periphery of tumor lesions. The sampled lesion has not received local therapy (e.g., radiotherapy, radiofrequency ablation, oncolytic virus, etc.) or such interventions have occurred ≥3 months prior and the lesion has progressed after local treatment;

• Expected life expectancy ≥3 months;

• After tumor resection/puncture, the subject must have at least one measurable lesion according to Response Evaluation Criteria in Solid Tumors (RECIST v1.1) for efficacy evaluation;

• Eastern Cooperative Oncology Group (ECOG) performance status was 0-1 (subjects with stable brain metastases require investigator assessment);

• Adequate organ function：

  1. Hematological (must meet the following criteria within 24 hours before apheresis/blood collection; no transfusions, platelet infusions, or growth factor support [except recombinant erythropoietin] within 7 days prior to enrollment):

     1. Absolute neutrophil count (ANC) ≥1 × 10⁹/L;
     2. Platelet count (PLT) ≥80 × 10⁹/L;
     3. Hemoglobin (Hb) ≥90.0 g/L;

  2. Blood chemistry:

     1. Estimated creatinine clearance ≥40 mL/min (calculated by Cockcroft-Gault formula);
     2. Alanine aminotransferase (ALT) ≤3 × upper limit of normal (ULN);
     3. Aspartate aminotransferase (AST) ≤3 × ULN;
     4. Total bilirubin (TBIL) ≤2 mg/dL (subjects with Gilbert-Meulengracht syndrome ≤3 mg/dL);

  3. Serum AST and ALT ≤5 × ULN (subjects with liver metastasis);

  4. Adequate pulmonary reserve defined as ≤Grade 1 dyspnea and oxygen saturation >91% on room air;

  5. Left ventricular ejection fraction (LVEF) ≥45% by echocardiography or multigated acquisition (MUGA) scan, with hemodynamic stability;

• In the investigator's judgment, the subject must have recovered from prior anticancer therapy toxicities to Grade 1 or lower (except for specific Grade 2 or lower toxicities deemed irreversible in a short period of time as judged by the investigator, e.g., alopecia) and be eligible for preconditioning chemotherapy and TIL therapy;

• Subjects with documented ≥Grade 2 diarrhea or colitis from prior immune checkpoint inhibitor therapy must be asymptomatic for ≥6 months before tumor resection, with normal colonoscopy (visual assessment) post-immunotherapy (excluding colorectal cancer patients);

• Subjects with immune-related endocrinopathies (e.g., hypothyroidism) may enroll if stable for ≥6 weeks and controlled with hormone replacement therapy (non-corticosteroid);

• Women of childbearing potential and all male subjects must agree to use highly effective methods of contraception at the time of informed consent, and continue within 1 year after Meta10-TILs infusion.

Exclusion Criteria:

• Active central nervous system (CNS) metastases (except for stable brain metastases not requiring medication or steroid dependence for ≥3 months).
• Use of Chinese herbal medicine or botanical drugs with antitumor indications within 1 week before preconditioning.
• Systemic corticosteroid therapy (≥10 mg/day prednisone or equivalent) or other immunosuppressive drugs within 2 weeks before preconditioning (excluding inhaled, topical, or physiological replacement therapy).
• Subjects who have undergone major surgery within 4 weeks before enrollment (as assessed by the investigator) or planned major surgery during the study (excluding scheduled surgery for Meta10-TILs preparation)；Major surgery refers to Grade 3 & 4 surgeries as defined by China's Administrative Measures for Clinical Application of Medical Technology (effective on May 1, 2009).
• History of other malignancies within 3 years before screening or concurrent malignancies (except for locally treated malignancies with no recurrence risk for ≥1 year, e.g., non-melanoma skin cancer, bladder cancer).
• Any form of primary immunodeficiency disorder (e.g., severe combined immunodeficiency [SCID] or acquired immunodeficiency syndrome [AIDS]).
• History of organ transplantation.
• Active hepatitis B (HBsAg positive or anti-HBc positive with HBV-DNA >1000 copies/mL) or hepatitis C (HCV-RNA positive).
• Anti-HIV antibody positive or anti-syphilis antibody positive.
• Uncontrolled acute life-threatening bacterial, viral, or fungal infections (e.g., positive blood culture ≤72 hours before Meta10-TILs infusion).
• Patients who have received a live or attenuated vaccination within 4 weeks before preconditioning.
• Unstable angina and/or myocardial infarction within 6 months before signing informed consent；Uncontrolled thrombotic events, severe bleeding, or deep vein thrombosis (DVT) within 12 months before signing informed consent.
• History of neurological or psychiatric disorders, including epilepsy or dementia.
• History of hypersensitivity to drugs (e.g., cyclophosphamide, fludarabine, IL-2, Meta10-TILs components, gentamicin, etc.).
• High bleeding risk per investigator assessment, including but not limited to: tumor encasement/infiltration of major blood vessels (e.g., carotid artery, jugular vein, bronchial artery)；other high-risk features (e.g., fistula, cavitary lesions, history of previous bleeding [≤60 days]).
• Patients who have received other investigational therapies within 30 days before signing informed consent.
• Other conditions deemed ineligible for the study by the investigator (e.g., Grade ≥3 adverse events in previous immunotherapy).