fMRI Evaluation of Auricular Acupuncture Targets: An Exploratory Clinical Study

Auricular Press-Needle Acupuncture Induces Specific Brain Functional Reorganization and Modulates Autonomic Nervous Activity: A Randomized Sham-Controlled Trial

This study employs resting-state functional magnetic resonance imaging (rs-fMRI) to examine whether auricular press needles acupuncture modulates functional connectivity between the insula and medial prefrontal cortex (MPFC), in a manner comparable to transcranial vagus nerve stimulation (taVNS), and to assess its association with interoceptive improvement. By establishing a neurophysiological baseline for auricular vagus nerve stimulation (aVNS) in the healthy brain, the research aims to clarify its regulatory mechanisms in cognition and emotion. The findings provide a key theoretical and evaluative framework for translating aVNS into clinical applications for insomnia and depression.

Inclusion Criteria:

1. Age 18-30 years;
2. Basically normal diet and sleep;
3. No history of mental illness;
4. No MRI contraindications (e.g., metal implants or pacemakers) or claustrophobia；
5. Willing to participate in this study and sign the informed consent form.

Exclusion Criteria:

1. Presence of auricular skin lesions or allergy to adhesive ear patches;
2. Currently receiving regular acupuncture treatment;
3. History of bleeding disorders or anticoagulant use (increased bleeding risk);
4. Previous history of syncope during acupuncture.