The goal of this clinical trial is to assess the effect of Nano-biofusion gingival gel (NBF) on wound healing at the palatal donor site following soft tissue graft surgery and compare it to placebo gel. The main questions it aims to answer are:

• Does NBF gel enhance wound healing after graft harvesting from the palate?
• Does NBF gel reduce the number of inflammatory mediators after surgery?
• Does NBF gel reduce pain and discomfort at the palatal donor site? Researchers will compare NBF gel to a placebo (a look-alike gel that does not contain the active ingredients) to see if NBF gel enhances wound healing.

Study steps include:

• Participants will apply the assigned gel on the surgical wound 4 times a day for 4 weeks.
• Clinical photographs and measurements will be obtained on days (0, 1, 2, 4, 7, 15, 21, 30).
• Wound fluid samples will be collected on days (0, 1, 2, 4, 7).
• Participants will be asked about the level of pain and analgesic consumption on each follow-up visit.

A sample size of 36 subjects, with treated 18 sites in each group should be sufficient to detect a mean difference of 1 mm of the wound area between groups. Efforts will be made to balance the number of men and women in each group (if possible).

The participants will be recruited from patients coming to dental teaching clinics at Jordan University of Science and Technology. The patients will be randomly assigned into 2 groups using computer generated randomization sequence:

Group 1: placebo gel Group 2: NBF gel

The study's purpose and procedures will be clearly communicated to all patient. Prior to examination and treatment, each participant must sign a consent form.

Patients who require a soft tissue surgery to cover gingival recession around teeth, to modify the phenotype where there is a thin gingival tissue, or to increase the width and thickness of soft tissue will be included in the study. The soft tissue graft should be obtained from the palatal donor site by free gingival graft or depithelized connective tissue graft, leaving an open wound in the palate (around 3×6 mm size) that is going to heal by secondary intesntion.

After the procedure, patients will be instructed to use the gel 4 times a day for 4 weeks by applying it to the wound and massaging the area. It will be provided in a small opaque tube. One tube should be used each day. The clinician and the patient will be blinded to the type of gel used. Another doctor or dental assistant will be responsible for dispencing the gel to the patient according to the randomization sequence and the code sheet.

Oral hygiene measures will be prohibited for the first 2 weeks in the surgical area only to not disturb the wound healing. Patients should follow their oral hygiene routine for the rest of the teeth. They will be instructed to use 0.2% chlorhexidine mouth wash twice a day for 2 weeks after surgery.

The study will last for one month for each patient. Each patient will be assessed immediately before and after the surgery and on days (0, 1, 2, 4, 7, 15, 21, 30). Clinical photographs will be taken at each time point.

Wound fluid samples (WF) will be collected at the incision area immediately after surgery and wound closure (day 0) and on days (1, 2, 4, 7). Main inflammotry mediators will be assessed.

Study steps:

• T0(intervention): Pt will sign the consent form. Sof tissue graft surgery will be performed and the graft will be obtained from the palate. The surgery is not performed by one periodontist, but the assessment and measurements are done by the same blinded examiner.

• T1 (24 hours after the surgery), T2 (2 days after the surgery), T3 (4 days after the surgery), T4 (7 days after the surgery), T5 (15 days after the surgery), T6 (21 days after the surgery), T7 (30 days after the surgery): The wound will be measured to record all the primary and secondary outcomes. The exception is made for the collection of wound fluid samples on days 15, 21 and 30. As the wound will be probably closed by then, samples will not be collected, unless there is delayed wound healing and the fluid can be drawn from the wound.

Clinical photos will be taken before surgery and anesthesia, immediately after surgery and the rest of time points.

Inclusion Criteria:

1. Patients with gingival recession that is indicated for root coverage or need for phenotype modification.
2. 18 years or older.
3. absence of systemic disease
4. Non-smoker
5. No use of antibiotics, corticosteroids, anti-inflammatory medication or hormones in the past 3 months

Exclusion Criteria:

1. Pregnant or lactating woman.
2. patients with immune or systemic diseases.
3. Patients with an allergy to any ingredient in the gel.

Individual participant data for wound size, early wound healing index, and patient-reported outcomes will be shared. Data will be stripped of all identifiers to protect participants privacy.

IPD and supporting documents will be available after publication of the main study results, for a period of one year.

Access will be granted to qualified researchers who request the data via email to the principal investigator. Researchers must sign a data use agreement ensuring ethical use, confidentiality, and prohibition of attempts to identify participants.

The gel will be provided in a small opaque tube. The clinician and the patient will be blinded to the type of gel used. Other doctor or dental assistant will be responsible for dispencing the gel to the patient according to the randomization sequence and the code sheet.