Acute Effects of Combined and Isolated Caffeine and Theanine Supplementation on Physical and Cognitive Performance in Competitive Athletes: A Randomized, Double-Blind, Placebo-Controlled Crossover Study
Acute Effects of Combined and Isolated Caffeine and Theanine Supplementation on Physical and Cognitive Performance in Competitive Athletes: A Randomized, Double-Blind, Placebo-Controlled Crossover Study
The aim of this clinical trial is to examine how caffeine and L-theanine, taken alone or together, affect both physical and cognitive performance in healthy, trained athletes. The study aims to answer whether these commonly consumed dietary ingredients can enhance strength, endurance, and hand-eye coordination under controlled laboratory conditions.
Researchers will compare four supplementation conditions:
Caffeine (CAF) - 3 mg per kilogram of body weight L-Theanine (THE) - 200 mg Combined Caffeine and L-Theanine (COM) - same doses together Placebo (CON) - mannitol (inactive substance) Each participant will complete all four test sessions in a randomized, double-blind, crossover design, with at least 72 hours between sessions. Capsules will be taken 60 minutes before testing to allow time for absorption. During each session, participants will perform a series of physical and cognitive tasks, including maximal isometric strength tests, an intermittent endurance test, and a computerized eye-hand coordination task.
The main hypothesis is that caffeine and L-theanine, alone or in combination, will improve strength and cognitive coordination compared with placebo. All participants are healthy competitive athletes between 18 and 30 years old. The study is conducted under standardized laboratory and indoor sports-hall conditions, with ethics approval obtained from the Sinop University Human Research Ethics Committee (Approval No. 2025/337).
This randomized, double-blind, placebo-controlled, crossover clinical trial investigates the acute effects of caffeine (CAF), L-theanine (THE), their combination (COM), and placebo (CON) supplementation on physical and cognitive performance in young, healthy, competitive athletes. Each participant completed all four experimental conditions in randomized order, separated by a minimum 72-hour washout period to minimize carryover effects. The randomization sequence was computer-generated by an independent researcher, and both participants and assessors were blinded to all conditions.
A total of 20 trained athletes (12 males, 8 females; 19.9 ± 2.4 years) participated. All were actively engaged in team or individual sports at a competitive level, with no history of cardiovascular, neurological, or musculoskeletal disorders, and no known intolerance to caffeine, theanine, or placebo components. Anthropometric and body composition data were collected using standardized procedures, including multi-frequency bioelectrical impedance analysis. Participants maintained normal caffeine consumption habits to preserve ecological validity and avoid withdrawal effects.
The supplementation protocol consisted of four conditions: (1) 3 mg/kg anhydrous caffeine, (2) 200 mg L-theanine, (3) a combination of both, and (4) placebo (mannitol). All supplements were encapsulated in visually identical gelatin capsules, and participants ingested five capsules with water 60 minutes before testing. Testing sessions were conducted at 09:00 a.m. to minimize circadian variability. Participants abstained from alcohol, strenuous exercise, and dietary supplements for 24 hours before each session, and replicated their pre-trial dietary intake based on 24-hour dietary logs.
All testing sessions were performed under standardized indoor laboratory conditions (22 ± 1 °C, 50 ± 5% humidity). The test sequence was fixed as eye-hand coordination, maximal isometric strength, and intermittent aerobic endurance, with adequate rest intervals between tests.
Primary outcomes included:
Maximal isometric strength, measured via handgrip, back, and leg dynamometry (Lafayette, Japan).
Intermittent aerobic endurance, assessed using the Yo-Yo Intermittent Endurance Test Level 1.
Eye-hand coordination, evaluated using a computerized test battery (EHC) that quantified accuracy, speed, and precision parameters.
A priori power analysis (f = 0.25, α = 0.05, 1-β = 0.80) determined a required sample size of 16 participants; 20 were recruited to allow for potential attrition. All 20 completed the study.
The study protocol was approved by the Sinop University Human Research Ethics Committee (Approval No. 2025/337), and written informed consent was obtained from all participants.
This trial aims to clarify whether acute ingestion of caffeine, L-theanine, or their combination exerts differential or synergistic effects on physical strength, endurance, and coordination in trained athletes, thereby contributing to the understanding of combined ergogenic aids in sports performance.
Inclusion Criteria:
Exclusion Criteria: