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| Name | Class |
|---|---|
| ETH Zurich | OTHER |
| Laboratory of Stem Cell Biology and Ageing, ETH Zurich, Prof. Nina Cabezas-Wallscheid | UNKNOWN |
| Prof. Dr. Nina Cabezas-Wallscheid, ETH Zurich | UNKNOWN |
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This study investigates how caffeine intake affects blood stem and progenitor cells in healthy adults.
The trial will compare people who regularly consume caffeine with those who consume very little or none. All participants will receive a single 200 mg caffeine tablet (similar to one cup of coffee) under fasting conditions. Blood samples will be collected before and three hours after caffeine intake.
The study will assess whether caffeine influences the mobilization of blood stem and progenitor cells from the bone marrow into the bloodstream. It will also examine the effects of caffeine on the function, gene activity, and metabolism of blood cells.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Caffeine intervention | Experimental | 200mg Caffeine |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Caffeine (200 mg) | Other | Participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after intake to evaluate effects on hematopoietic stem and progenitor cells, including mobilization, function, gene expression, and metabolism. |
| Measure | Description | Time Frame |
|---|---|---|
| Mobilization of hematopoietic stem and progenitor cells | The change in the numbers of mobilized HSPCs (defined as CD34+ cells) [cells/ml] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers. | From baseline to three hours after caffeine intake |
| Mobilization of hematopoietic stem and progenitor cells | The change in the relative abundance of HSPCs [% HSPCs of total PBMCs] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers. | From baseline to three hours after caffeine intake |
| Measure | Description | Time Frame |
|---|---|---|
| Functional alterations in CD34+ peripheral blood cells | Functional alterations in CD34+ PB cells: self-renewal [number of colonies] assessed via colony-forming unit assay. | From baseline to three hours after caffeine intake |
| Functional alterations in CD34+ peripheral blood cells |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| ETH GLC | Not yet recruiting | Zurich | Canton of Zurich | 8006 | Switzerland |
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| ID | Term |
|---|---|
| D002110 | Caffeine |
| ID | Term |
|---|---|
| D014970 | Xanthines |
| D000470 | Alkaloids |
| D006571 | Heterocyclic Compounds |
| D011688 | Purinones |
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This is a single-group, open-label study in which all participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after caffeine intake to assess mobilization, function, gene expression, and metabolic profile of hematopoietic stem and progenitor cells.
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Functional alterations in CD34+ PB cells: single HSPC proliferation [number of cell divisions within 72h] assessed via single HSC proliferation assay. |
| From baseline to three hours after caffeine intake |
| Transcriptional changes in CD34+ cells | Transcriptional changes in CD34+ cells [log2 fold change in gene expression and DEG count] assessed via single-cell RNA sequencing. | From baseline to three hours after caffeine intake |
| Relative metabolite abundance | Relative metabolite abundance [arbitrary units based on peak area or height in LC-MS] in plasma and intracellularly in isolated CD34+ cells. | From baseline to three hours after caffeine intake |
| Change in the numbers of peripheral blood cells | The change in the numbers of PB cells [cells/ml], including B cells, T cells, myeloid cells in blood, and relative, and measured by flow cytometry | From baseline to three hours after caffeine intake |
| Change in the numbers of peripheral blood cells | The change in relative abundance of PB cells [% cell type of total PBMCs] including B cells, T cells, myeloid cells in blood, and measured by flow cytometry | From baseline to three hours after caffeine intake |
| ETH GLC | Recruiting | Zurich | Canton of Zurich | 8006 | Switzerland |
|
| D011687 |
| Purines |
| D006574 | Heterocyclic Compounds, 2-Ring |
| D000072471 | Heterocyclic Compounds, Fused-Ring |