Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
This study aims to examine the effects of acute aerobic exercise and cardiorespiratory fitness on cellular metabolism of CD8+ T cells and regulatory T cells (Tregs) found in the peripheral blood of humans. In addition, the study will investigate whether the effects of exercise differ based on exercise intensity, as well as whether the effects of exercise and fitness differ between subpopulations of CD8+ T cells and Tregs. Finally, the study aims to examine whether exercise- and fitness-induced changes in cell metabolism relate to changes in cell function.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Acute effects: Moderate Intensity Exercise | Experimental | Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will cycle 30minutes exercise below ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized. |
|
| Acute effects: Heavy intensity exercise | Experimental | Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will bicycle 30 minutes above ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Acute exercise-moderate intensity | Behavioral | 30 minutes of cycling exercise at moderate (90% of first ventilatory threshold) |
|
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic Function | Cellular metabolic activity and poise of T cells as measured by flow cytometry (SCENITH) | At rest, immediately after, and two hours after exercise |
| Immune Cell function | Stimulated IFN-gamma, perforin and IL-10 production, as measured by flow cytometry | At rest, immediately after, and two hours after exercise |
| Metabolic Regulation | Expression of genes involved in cellular energy metabolism, as assessed by RT-PCR | At rest, immediately after, and two hours after exercise |
| Mitochondrial Regulation | Mitochondrial mass and membrane potential, as assessed by flow cytometry | At rest, immediately after, and two hours after exercise |
| Measure | Description | Time Frame |
|---|---|---|
| Peripheral immune cell composition | Number (concentration) of immune cell subsets in peripheral blood, as measured by hematology analyzer and flow cytometer | At rest, immediately after, and two hours after exercise |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Houston | Houston | Texas | 77204 | United States |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Acute effects-heavy intensity | Behavioral | Participants will cycle 30 minutes at heavy (110% of first ventilatory threshold) intensity |
|