The goal of this clinical trial is to learn tissue flossing and proprioceptive neuromuscular facilitation effect in treating hamstring muscle tightness.

The main questions it aims to answer are:

1. which technique is more useful in giving instant relaxation of hamstring muscle
2. can these technique increase muscle flexibility and knee motion

Researchers will compare Tissue flossing to a PNF (Proprioceptive neuromuscular facilitation) to see which on is more effective.

Participant will:

1. be provided one technique either tissue flossing or PNF in first day
2. physiotherapist will immediately measure the the change in flexibility or knee motion

2. another technique will be applied after 1 week 3. again change in flexibility and knee motion will be recorded

Inclusion Criteria:

• Female subjects were included in the study
• Subjects with hamstring muscle tightness were included
• Subjects between the age group of 18-25 years were included

Exclusion Criteria:

• Male subjects
• Subject who didn't fulfill the age criteria
• Subject with hamstring tear or other knee ligament injury
• Any history of lower limb surgery dating back a year
• Subjects with Lower back pain or any neurological compromise in the lower limb
• Any acute injury like a sprain, strain, and open wound
• Subjects who had latex allergies
• Subjects with venous thrombotic disease, heart disease, respiratory illness, and dermatitis, in their lower legs were excluded

Only female participants were eligible for inclusion in this study. The study focused on young women because sex-related differences in hamstring flexibility, joint range of motion, and neuromuscular properties have been reported in the literature. Restricting the sample to female students provided a more homogeneous group and reduced variability related to gender differences, thereby enhancing internal validity

I did not include individual participant data in ClinicalTrials.gov to protect participant privacy and confidentiality.