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Chronic wounds and impaired healing remain a major clinical challenge, particularly in patients with diabetes, vascular disease, or age-related skin fragility. Low-level laser therapy (LLLT) at 660 nm has been reported to enhance tissue repair, reduce inflammation, and promote angiogenesis. However, the biomechanical properties of wound tissue following LLLT remain poorly understood.
Optical Coherence Tomography (OCT) provides a non-invasive, high-resolution method to assess skin microstructure and elasticity, while the MyotonPRO offers quantitative stiffness measurements at the tissue surface. Combining these two complementary modalities may provide a more comprehensive assessment of wound healing progression and treatment response.
This study aims to evaluate changes in wound stiffness before and after 660 nm LLLT and to compare them against contralateral normal skin, thereby providing objective insight into the biomechanical efficacy of laser treatment.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Skin status (normal, wound, after treatment) | Active Comparator | Using 660 nm LLLT efficacy on the wound, separated categories into the upper limb and lower limb. |
|
| Skin location (upper limb, lower limb) | Active Comparator | Using 660 nm LLLT efficacy on the wound, separated categories into the upper limb and lower limb. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Low Level Laser Therapy | Radiation | Collecting wound stiffness data using the OCT-air-jet and Myoton Pro before and after the LLLT treatment using the device's indentation system. Then the intervention is applying LLLT to the wound for 3 min. The LLLT utilized the Red Dot Laser Diode Module Class IIIA. |
| Measure | Description | Time Frame |
|---|---|---|
| To evaluate changes in skin stiffness of wound tissue before and after LLLT using OCT and MyotonPRO. | 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| To compare wound skin stiffness with contralateral normal skin. | To assess differences in stiffness response between upper-body and lower-body wound sites. To determine the correlation between OCT and MyotonPRO stiffness measurements. | 1 year |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Asia University | Taichung | Wufeng | 41354 | Taiwan |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 42252133 | Derived | Ramadhan GT, Jan YK, Liau BY, Cheng HT, Shen WC, Tai CC, Pravin SC, Venkataramani K, Lee WC, Ching CTS, Lung CW. Deep Learning-Based OCT Segmentation for Stiffness Quantification in Evaluating Low-Level Laser Therapy for Wound Healing. J Biophotonics. 2026 Jun;19(6):e70291. doi: 10.1002/jbio.70291. |
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| ID | Term |
|---|---|
| D014947 | Wounds and Injuries |
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| ID | Term |
|---|---|
| D028022 | Low-Level Light Therapy |
| ID | Term |
|---|---|
| D053685 | Laser Therapy |
| D013812 | Therapeutics |
| D010789 | Phototherapy |
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