Evaluation of Skin Stiffness Changes in Wounds Before and After Low-Level Laser Therapy

Evaluation of Skin Stiffness Changes in Wounds Before and After Low-Level Laser Therapy Using 660 nm Laser: A Comparative Analysis With OCT and MyotonPRO

Chronic wounds and impaired healing remain a major clinical challenge, particularly in patients with diabetes, vascular disease, or age-related skin fragility. Low-level laser therapy (LLLT) at 660 nm has been reported to enhance tissue repair, reduce inflammation, and promote angiogenesis. However, the biomechanical properties of wound tissue following LLLT remain poorly understood.

Optical Coherence Tomography (OCT) provides a non-invasive, high-resolution method to assess skin microstructure and elasticity, while the MyotonPRO offers quantitative stiffness measurements at the tissue surface. Combining these two complementary modalities may provide a more comprehensive assessment of wound healing progression and treatment response.

This study aims to evaluate changes in wound stiffness before and after 660 nm LLLT and to compare them against contralateral normal skin, thereby providing objective insight into the biomechanical efficacy of laser treatment.

Inclusion Criteria:

• presented chronic and burn wounds lasting more than four weeks.
• had partial-thickness burn wounds.

Exclusion Criteria:

• current smokers.
• wounds that were fresh and still bleeding.