Surgical extraction of impacted third molar without autologous albumin gel application.

Surgical extraction of impacted third molar with autologous albumin gel application.

Socket curettage and bone filing will be done using a bone curette and bone file, respectively.

Extraction sockets with albumin gel application. 0.2 ml of autologous albumin gel will be applied to all four edges of the extraction socket

Measurements will be taken before surgery (baseline). Swelling will be evaluated by comparing the difference between preoperative and postoperative measurements. Swelling will be evaluated in Centimeters using a tape measure.

Performed on the patient's operated site using these reference points:

• A) Lateral corner of the eye/angle of the mandible
• B) Tragus/outer corner of the mouth
• C) Tragus/soft tissue pogonion

The area of operation will be examined for Any manifestations of wound healing disturbance including:

• Swelling, redness, hotness, pus discharge, signs and symptoms of infection, Wound dehiscence
• Exposure of underlying hard tissue will be carefully noted

Pain will be evaluated using Visual Analogue Scale (VAS). Which is a 10 point scale, in which the minimum end '0" represents no pain, while the maximum end 10" represents the worst pain.

First 5 cm of blood will be withdrawn under aseptic conditions using sterile venipuncture, then blood will be separated immediately by centrifugation (3000 rpm). To be assessed using enzyme-linked immunosorbent assays (ELISA) kits according to the manufacturer's protocol for their Serum concentrations of: interleukin 6, macrophage inflammatory protein, granulocyte macrophage colony-stimulation factor, and vascular endothelial growth factor