Not provided
Not provided
| ID | Type | Description | Link |
|---|---|---|---|
| SIATCAS | Other Identifier | Shenzhen Institutes of Advanced Technology，Chinese Academy of Sciences |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The goal of this observational study is to learn about the effects of physical activity in adult on metabolic response. The main question it aims to answer is:
Participants will engage in the following measurements:
The purpose of this study is to explore the effect of various physical activities on metabolic and behavior response. Energy expenditure, body composition, and metabolism will be measured by the following measurements:
TEE will be measured using the DLW method. Urine samples from all participants in the DLW subset will be stored at -20℃ and shipped on dry ice for analysis in the laboratory of Prof. John Speakman at the Shenzhen Institutes of Advanced Technology, Chinese academy of sciences. Isotopes will be measured in benchtop near-infrared isotope gas analyzer, and mean CO2 production will be calculated from isotope ratios using the recently derived equation. TEE will then be calculated using mean CO2 production.
REE and TEF will be measured by indirect calorimetry while participants are resting supine in a respiratory chamber with thermoneutral conditions. Oxygen consumption and CO2 production will be measured to calculate energy expenditure. REE will be consistently measured throughout sleeping at night and in the morning after overnight fasting, and TEF will be measured after breakfast.
Physical activity of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 14 days. The monitor should not be worn while bathing or swimming. The first day is discarded along with any day with less than 12 hours of wear. For a valid measure the goal is to get 2 weekday and 2 weekend days.
Participants need to warm up with simple stretching or light aerobic exercise before the test. The test will be conducted on a treadmill. A comfortable running speed acceptable to the participants will be set. The treadmill's incline will be increased by 10% every two minutes until the individual reaches their physical limit and can no longer continue. During this test, a respiratory mask and gas analyzer will be used to measure the concentration of oxygen (O2) and carbon dioxide (CO2) in the breath to calculate VO2max.
Photographic and food weighing methods to record food intake for two random days throughout the period of DLW measurement.
Fasting and post-prandial glucose will be recorded by a continuous glucose monitoring system.
Fat mass will be measured by Magnetic Resonance Imaging (MRI, Shanghai united imaging, uMR 790 ).
Bioimpedance Analysis will measure fat-free mass (Tanita, MC-980). Bone mass will be measured by Dual Energy X-ray Absorptiometry (DXA, Horizon Wi, HOLOGIC).
At visit 1, fasting body weight will be measured using a calibrated seca at the beginning of the visit. Fasting weight will be measured at visit 2 and, if applicable.
Height (to +0.1 cm) will be measured by seca 217 stable without wearing shoes.
Hip and waist circumference (to +0.3 cm) will be measured using a whole body laser scanner.
Systolic and diastolic blood pressure will be measured using an Omron digital sphygmomanometer.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sedentary group | Godin scale score ≥ 24 |
| |
| Moderately active group | 14≥ Godin scale score ≥ 23 |
| |
| Active group | Godin scale score <14 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Observation without intervention | Other | Observation without intervention |
|
| Measure | Description | Time Frame |
|---|---|---|
| Differences in total energy expenditure (TEE) measured by the doubly labeled water (DLW) method across experimental groups | - The change in TEE will be evaluated using the DLW. Urine samples from all participants in the DLW subset will be stored at -20℃ and shipped on dry ice for analysis in the laboratory of Dr. John Speakman at the University of Aberdeen, or the Shenzhen Institutes of Advanced Technology, Chinese academy of sciences. Isotopes will be measured in benchtop near-infrared isotope gas analyzer, and mean CO2 production will be calculated from isotope ratios using the recently derived equation (Speakman et al 2021: Cell reports medicine). TEE will then be calculated using mean CO2 production using the Weir equation. | 14days |
| Differences in non-exercise physical activity across experimental groups | - Physical activity of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 14 days. | 14days |
| Differences in resting energy expenditure (REE) measured by respiratory chamber across experimental groups | - REE will be evaluated by respiratory chamber. | 1 day |
| Differences in thermic effect of food (TEF) measured by respiratory chamber across experimental groups | - TEF will be evaluated by respiratory chamber. | 1 day |
| Differences in exercise energy expenditure (EEE) measured by COSMED across experimental groups | - EEE will be evaluated using an indirect calorimetry COSMED. | 1 hour |
| Differences in food intake across experimental groups |
| Measure | Description | Time Frame |
|---|---|---|
| Differences in sedentary time measured by GT3X accelerometer across experimental groups | - Sedentary time will be represented from the GT3X accelerometer. | 14 days |
| Differences in glucose across experimental groups |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Residents of Shenzhen, Guangzhou, China
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| John R Speakman, PhD | Contact | +8615810868669 | j.speakman@abdn.ac.uk | |
| Xinyi BI, PhD | Contact | +8614774821721 | xy.bi@siat.ac.cn |
| Name | Affiliation | Role |
|---|---|---|
| John R Speakman, PhD | Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Shenzhen Institutes of Advanced Technology，Chinese Academy of Sciences | Shenzhen | Guangdong | China |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33665639 | Result | Speakman JR, Yamada Y, Sagayama H, Berman ESF, Ainslie PN, Andersen LF, Anderson LJ, Arab L, Baddou I, Bedu-Addo K, Blaak EE, Blanc S, Bonomi AG, Bouten CVC, Bovet P, Buchowski MS, Butte NF, Camps SGJA, Close GL, Cooper JA, Creasy SA, Das SK, Cooper R, Dugas LR, Ebbeling CB, Ekelund U, Entringer S, Forrester T, Fudge BW, Goris AH, Gurven M, Hambly C, El Hamdouchi A, Hoos MB, Hu S, Joonas N, Joosen AM, Katzmarzyk P, Kempen KP, Kimura M, Kraus WE, Kushner RF, Lambert EV, Leonard WR, Lessan N, Ludwig DS, Martin CK, Medin AC, Meijer EP, Morehen JC, Morton JP, Neuhouser ML, Nicklas TA, Ojiambo RM, Pietilainen KH, Pitsiladis YP, Plange-Rhule J, Plasqui G, Prentice RL, Rabinovich RA, Racette SB, Raichlen DA, Ravussin E, Reynolds RM, Roberts SB, Schuit AJ, Sjodin AM, Stice E, Urlacher SS, Valenti G, Van Etten LM, Van Mil EA, Wells JCK, Wilson G, Wood BM, Yanovski J, Yoshida T, Zhang X, Murphy-Alford AJ, Loechl CU, Melanson EL, Luke AH, Pontzer H, Rood J, Schoeller DA, Westerterp KR, Wong WW; IAEA DLW database group. A standard calculation methodology for human doubly labeled water studies. Cell Rep Med. 2021 Feb 16;2(2):100203. doi: 10.1016/j.xcrm.2021.100203. eCollection 2021 Feb 16. | |
| 26832439 |
| Label | URL |
|---|---|
| A standard calculation methodology for human doubly labeled water studies. | View source |
Not provided
Not provided
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
Not provided
Not provided
| ID | Term |
|---|---|
| D019370 | Observation |
| D008722 | Methods |
| ID | Term |
|---|---|
| D008919 | Investigative Techniques |
Not provided
Not provided
Not provided
Not provided
Not provided
Photographic and food weighing methods to record food intake for two random days throughout the period of DLW measurement
| 2 days |
Fasting and post-prandial glucose will be recorded by a continuous glucose monitoring system.
| 7 days |
| Differences in fat mass across experimental groups | Fat mass will be measured by Magnetic Resonance Imaging (Shanghai united imaging, uMR 790 ). | 10 minutes |
| Differences in fat-free mass across experimental groups | Fat-free mass will be measured by Bioimpedance Analysis (Tanita, MC-980). | 2 minutes |
| Differences in bone mass intensity across experimental groups | Bone mass intensity will be measured by DXA( Horizon Wi, HOLOGIC) | 7 minutes |
| Differences in circulating hormones across experimental groups | The nurse will collect the fasting blood sample. Metabolomics will analyze circulating hormones and metabolites. | 1 minutes for collection, 7 days for analysis. |
| Differences in intestinal microbiota across experimental groups | Feces will be collected for gut microbiome analysis of intestinal microbiota. | 5 minutes for collection, 7 days for analysis. |
| Result |
| Pontzer H, Durazo-Arvizu R, Dugas LR, Plange-Rhule J, Bovet P, Forrester TE, Lambert EV, Cooper RS, Schoeller DA, Luke A. Constrained Total Energy Expenditure and Metabolic Adaptation to Physical Activity in Adult Humans. Curr Biol. 2016 Feb 8;26(3):410-7. doi: 10.1016/j.cub.2015.12.046. Epub 2016 Jan 28. |
| Constrained Total Energy Expenditure and Metabolic Adaptation to Physical Activity in Adult Humans. | View source |