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This is a retrospective study with a sibling design comparing new oocyte thawing protocols (rapid and ultra-rapid) with the traditional method
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Standard | Other | Standard thawing |
|
| Fast | Other | Fast thawing |
|
| Ultrafast | Other | Ultrafast thawing |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Thawing protocol | Other | Standard thwaing |
| |
| thawing protocol |
| Measure | Description | Time Frame |
|---|---|---|
| Oocytes Survival rate | Oocyte survival rate will be assessed 2 hours after thawing using morphological evaluation under a light microscope. Surviving oocytes will be identified by the presence of an intact zona pellucida and oolemma, a round shape, and homogeneous cytoplasm without signs of lysis, fragmentation, or cytoplasmic granulation. | 2 hours post-warming |
| Measure | Description | Time Frame |
|---|---|---|
| Spindle configuration | Spindle configuration in thawed oocytes will be assessed using confocal microscopy following immunofluorescence staining. The spindles will be classified as normal, slightly aberrant, or aberrant. | 2 hours post thawing |
| Chromosome distribution |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| IRCCS San Raffaele Hospital | Milan | Lombardy | 20132 | Italy |
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sibling design
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| Other |
Fast thawing |
|
| thawing protocol | Other | Ultrafast thawing |
|
Chromosome distribution will be assessed using confocal microscopy following immunofluorescence staining. The distribution will be classified as normal, misaligned, or displaced. |
| 2 hours post thawing |