This study aims to investigate the association between periodontitis Stage III and IV and the intensity and duration of sleep bruxism episodes. Surface electromyography (EMG) of the masseter muscle will be used to instrumentally assess sleep bruxism in patients with and without periodontal disease

The purpose of this cross-sectional study is to evaluate whether patients with Stage III and IV periodontitis exhibit differences in sleep bruxism activity compared to individuals without periodontal disease. Participants will undergo comprehensive periodontal assessment and 24-hour EMG recording of the masseter muscle using a portable device. Bruxism activity will be quantified using the Bruxism Work Index (BWI) and Bruxism Time Index (BTI). The study aims to clarify the potential relationship between periodontal inflammation and masticatory muscle activity during sleep, following standardized diagnostic and recording protocols

Inclusion Criteria:

• Age between 28 and 50 years
• Ability to perform adequate oral hygiene
• Willingness to participate and sign informed consent
• For periodontitis group: Diagnosis of Stage III or IV periodontitis
• For control group: Absence of periodontal disease

Exclusion Criteria:

• Use of removable dentures
• Ongoing orthodontic treatment
• Temporomandibular disorders (TMD) or orofacial pain
• Use of medications affecting muscle activity
• Systemic or psychiatric disorders
• Diabetes

Adult patients and healthy controls aged 28-50 years recruited at the University of Siena, Italy. The study includes both individuals with advanced periodontitis and healthy controls.