This randomized controlled trial aims to evaluate the effectiveness of a mobile- and virtual reality-based mindfulness and breathing intervention on stress, burnout, sleep quality, and cognitive function among Rotating-shift nurses. Participants will be randomly assigned to one of four groups: (1) health education control group, (2) mobile-based mindfulness only, (3) mobile-based mindfulness combined with brief structured breathing, and (4) virtual reality-assisted mindfulness combined with brief structured breathing. The intervention will last for 8 weeks, with participants practicing 5 times per week for 10 minutes per session. Primary outcomes include perceived stress, burnout levels, sleep quality, and cognitive function.

Rotating-shift nurses often experience high levels of chronic stress, occupational burnout, and disrupted sleep patterns, which can negatively affect their cognitive function and well-being. This study aims to investigate the effectiveness of technology-assisted mindfulness and breathing interventions in addressing these issues.

A total of four groups will be included in this randomized controlled trial:

1. Control Group (CG): Participants will receive a weekly electronic health education manual for 8 weeks. Topics include healthy eating habits, balanced nutrition, the physiological impact of shift work, sleep hygiene for shift workers, the effects of electronic devices on sleep, and identifying sources of stress. No mindfulness or breathing content is included.
2. Mobile-based Mindfulness Group (MMG): Participants will engage in guided mindfulness practice via a smartphone application, using a pre-recorded 360-degree nature video.
3. Mobile-based Mindfulness with Breathing Group (MMBG): Participants will follow a similar protocol as MMG, with the addition of brief structured breathing exercises embedded in the training.
4. Virtual Reality-based Mindfulness with Breathing Group (VMBG): Participants will wear a VR headset and immerse themselves in a virtual nature setting while engaging in combined mindfulness and structured breathing training. All intervention groups will complete 10-minute sessions, five times per week, for a total of eight weeks.

Primary outcome measures include: Perceived stress (Perceived Stress Scale, PSS-10-C), Occupational burnout (Nursing Burnout Scale), Sleep quality (Pittsburgh Sleep Quality Index, PSQI) and Cognitive function (including attention and memory tasks). Assessments will be conducted at baseline, immediately post-intervention, and at follow-up. This study will provide insights into the comparative effectiveness of mobile and immersive digital interventions for improving mental health and physiological functioning in Rotating-shift nurses.

Inclusion Criteria:

1. Age between 20 and 65 years.
2. Able to understand spoken or written Mandarin or Taiwanese.
3. Employed full-time for at least 3 months with a signed employment contract.
4. Has worked rotating shifts (including day, evening, and/or night shifts) in the past 3 months.
5. Willing to participate and sign informed consent.
6. Registered nurse.
7. A perceived stress score greater than or equal to 50 based on a standardized scale.

Exclusion Criteria:

1. Pregnant or breastfeeding individuals.
2. History of substance abuse (e.g., tobacco, illicit drugs).
3. Alcohol consumption ≥350 ml/week with alcohol content ≥3.5%.
4. Diagnosed or suspected autonomic nervous system disorders.
5. History of severe or unstable cardiovascular disease, cerebrovascular disease, cancer, or end-stage organ failure within the past 6 months.
6. Current diagnosis of psychiatric disorders (e.g., major depressive disorder, schizophrenia), or use of psychiatric or sleep medications (e.g., antidepressants, sedatives).
7. Reported discomfort (e.g., dizziness, nausea) when using virtual reality devices.
8. Engaged in other regular exercise programs, light therapy, or mind-body interventions during the study period.
9. Participating in other clinical trials simultaneously.

This is an open-label trial. Due to the nature of the behavioral interventions and the use of virtual reality or mobile devices, participants and implementers are aware of the assigned group. However, outcome assessors and data analysts will be blinded to group allocation to reduce assessment bias.