Ovarian PRP Injections for Improved Embryo Quality Using Embryo Quality Score

Ovarian Platelet-Rich Plasma (oPRP) Injections Improvement In IVF Patients Based On Time-Lapse Incubator Culture for Automated Tracking and AI for Embryo Quality Assessment : A Non-Randomized Propsective Self-Controlled Interventional Study

Patients will have ovarian PRP treatments and embryo development will be observed using time-lapse imaging and AI software.

. oPRP Treatment & Second IVF Cycle (Study Cycle)

• Following the first IVF cycle, patients will undergo their first oPRP injection:

  • Timing: 1-2 weeks after the onset of the next menstrual cycle.
  • Procedure: Under IV sedation, a patient's autologous PRP will be prepared and injected directly into both ovaries under transvaginal ultrasound guidance.

• Second IVF Cycle (oPRP Study Cycle)

  • The same ovarian stimulation protocol will be used for consistency.
  • A second oPRP injection will be performed on stimulation days 2-5 to enhance folliculogenesis.
  • Ovulation will be triggered at the same follicular size thresholds, and oocyte retrieval will follow identical protocols.
  • Fertilization methodology will be identical to the first cycle unless no fertilization is identified in the first cycle.
  • Embryo Development Analysis: Up to 15 embryos will be cultured in the AI incubator, where time-lapse imaging will track their morphokinetics, and AI-based analysis will assess embryo viability and implantation potential.

Inclusion Criteria:

• No none contraindications to IVF or PRP treatment
• Adequate ovarian reserves and AMH level
• Willingness to undergo two IVF cycles and oPRP treatment

Exclusion Criteria:

• Anovulation due to menopause or perimenopause
• Any health conditions that are contraindicated for IVF or oPRP treatment