Introduction: Despite the awareness of the importance of sleep in our lives, especially for athletes, the consequences of poor rest on sports performance are not well understood. Furthermore, the interaction between complete and partial sleep deprivation over one night and sleeping the usual hours for each person remains unclear.

Objectives: The aim of this study is to evaluate the effects of sleep deprivation on maximal fat oxidation (MFO) in male and female participants.

Inclusion Criteria:

• Inclusion Criteria:

  • Age between 18 and 35 years.
  • Body Mass Index (BMI) < 25 kg/m².
  • Resistance-trained individuals (more than 2 years of structured training).
  • Healthy men and women without neurological, cardiometabolic, immunological, or physical conditions that prevent them from exercising.
  • Participants must be able to perform the tests described in the following section.

Exclusion Criteria:

• History of neuromuscular diseases, heart disease, or conditions that may affect liver or muscle metabolism.
• Use of drugs, stimulants, or sports supplements that could interfere with the dietary supplement used in the study.
• Sedentary habits (less than 150 minutes/week of moderate exercise).
• Having undergone prolonged periods of physical inactivity in the 6 months prior to the study.
• Engaging in strenuous exercise within 48 hours prior to the tests.
• Failure to replicate the same food intake on both experimental days.
• Consumption of caffeine or any other stimulant the day before the tests.

Statistician