Intralesional bevacizumab injection for the treatment of keloid

Intralesional triamcinolone acetonide injection for the treatment of keloids

anti-VEGF

Six characteristics of the scar were assessed. These are: vascularity, height, pliability, and pigmentation, pain and pruritis. Each characteristic is given a score, which are added together to give an overall score between 0 and 18. Higher scores mean a worse outcome.

Evaluation of skin erythema index was done using a spectrophotometry device

Tissue levels of VEGF, collagen type 1 and 3 measured by Enzyme linked immunosorbent assay

Tissue levels of collagen type 1 and 3 measured by Enzyme linked immunosorbent assay

Two mm punch biopsies were obtained per area at baseline and one month after the last treatment session. The post-treatment biopsy 3as taken from the vicinity of the baseline biopsy. Tissues are fixed in 10% buffered formalin, embedded in paraffin and sectioned into 5 μm-thick sections. Specimens were stained with hematoxylin and eosin (H&E) and orcein (or demonstration of elastic fibers). Histological examination was carried out under a light microscopy. Assessment was done as follows:

1. Assessment of the collagen fibers will be done by examining H & E slides, putting grading from 1 to 4 according to the thickness and orientation of the collagen fibers and the spaces in between the fibers.
2. Assessment of the elastic fibers will be done through examining orcein stained specimens and grading the elastic fibers in the scars from 1 to 4 based on presence or absence of the elastic fiber and degree of degradation and fragmentation.