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Colorectal cancer (CRC) is a leading cause of mortality in China, with metastasis significantly contributing to poor outcomes. Histopathological growth patterns (HGPs) in colorectal liver metastasis (CRLM) provide vital prognostic insights, yet the limited number of pathologists highlights the need for auxiliary diagnostic tools. Recent advancements in artificial intelligence (AI) have demonstrated potential in enhancing diagnostic precision, prompting the development of specialized AI models like COFFEE to improve the classification and management of HGPs in CRLM patients. This study aims to develop and validate a Transformer-based deep learning model, COFFEE, for the classification of colorectal cancer subtypes using whole slide images (WSIs) from patients diagnosed with colorectal cancer liver metastasis. The model is pre-trained using self-supervised learning (DINO) on WSIs from the TCGA-COAD cohort, utilizing a Vision Transformer (ViT) architecture to extract 384-dimensional feature vectors from 256×256 pixel patches. The COFFEE model integrates a Transformer-based Multiple Instance Learning (TransMIL) framework, incorporating multi-head self-attention and Pyramid Position Encoding Generator (PPEG) modules to aggregate spatial and morphological information. The study includes training, testing, and prospective validation cohorts and evaluates the performance of the model in both binary and multi-class classification settings, as well as its potential to assist pathologists in clinical workflows.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Surgical pathology slides from the SAHSYSU, 1,994 WSIs from 297 slides dated July 3, 2013. | This group includes 297 patients with colorectal cancer liver metastasis (CRLM), from which 1,994 whole slide images (WSIs) were collected. These slides were used for developing and testing the COFFEE AI model for histopathological growth pattern (HGP) classification, providing valuable insights for tumor characterization and prognosis. |
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| Surgical pathology slides from the SAHSYSU , 972 WSIs from 104 patients dated April 21, 2023. | This cohort contains 104 patients diagnosed with CRLM. 972 WSIs were collected to validate the COFFEE model on a more recent dataset, evaluating the model's performance in both binary and four-class HGP classifications. |
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| Surgical pathology slides from the SAHSYSU, 114 WSIs from 30 patients dated 2024. | This prospective cohort consists of 30 patients with CRLM, from which 114 WSIs were obtained in 2024. The cohort was used to assess the clinical applicability of the COFFEE AI model through a prospective trial, comparing the diagnostic performance of pathologists with and without AI assistance. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| CRLM surgery | Procedure | Surgical resection of colorectal cancer liver metastasis (CRLM) involves the removal of metastatic lesions from the liver. This procedure is aimed at improving survival rates and reducing tumor burden in patients diagnosed with CRLM. The resection is performed to treat liver metastasis, and clinical outcomes, such as progression-free survival (PFS) and overall survival (OS), are assessed post-surgery to determine treatment efficacy. |
| Measure | Description | Time Frame |
|---|---|---|
| Classification Accuracy (%) of the COFFEE AI Model in Binary Identification of Histopathological Growth Patterns (HGPs) in CRLM Using Whole Slide Images | This outcome measures the diagnostic classification accuracy of the COFFEE AI model in detecting histopathological growth patterns (HGPs) in patients with colorectal cancer liver metastasis (CRLM). Accuracy is defined as the proportion of correctly predicted HGP labels compared to the ground truth labels determined by consensus of expert pathologists. The analysis includes binary classification (desmoplastic vs. non-desmoplastic). Accuracy will be calculated as: Accuracy = Total number of predictions / Number of correct predictions×100%. The outcome will be assessed using digital whole slide images obtained from liver metastasis specimens collected during surgery. Model performance will be evaluated 6 months post-surgery in a prospective validation cohort. | 6 months post-surgery (for prospective cohort) |
| Measure | Description | Time Frame |
|---|---|---|
| Classification Accuracy (%) of the COFFEE AI Model in Multi-Class Identification of Histopathological Growth Patterns (HGPs) in CRLM Using Whole Slide Images | This outcome measures the diagnostic classification accuracy of the COFFEE AI model in detecting histopathological growth patterns (HGPs) in patients with colorectal cancer liver metastasis (CRLM). Accuracy is defined as the proportion of correctly predicted HGP labels compared to the ground truth labels determined by consensus of expert pathologists. The analysis includes four-class classification (desmoplastic, replacement, pushing, and mixed). Accuracy will be calculated as: Accuracy = Total number of predictions / Number of correct predictions×100%. The outcome will be assessed using digital whole slide images obtained from liver metastasis specimens collected during surgery. Model performance will be evaluated 6 months post-surgery in a prospective validation cohort. |
| Measure | Description | Time Frame |
|---|---|---|
| Progression-Free Survival (PFS, in months) in Colorectal Cancer Liver Metastasis (CRLM) Patients Stratified by AI-based Histopathological Growth Pattern (HGP) Classification | This outcome evaluates the association between AI-based HGP classification (desmoplastic and non-desmoplastic) and progression-free survival (PFS) in patients with colorectal cancer liver metastasis (CRLM) following curative-intent resection. PFS is defined as the time from surgery to disease progression or death from any cause. Kaplan-Meier analysis will be used to estimate PFS for each HGP group, with comparisons by log-rank test. Multivariate Cox regression models will assess the prognostic value of HGPs, adjusting for clinical covariates (e.g., age, sex, metastasis number/size, chemotherapy, margin status, tumor burden score). Hazard ratios with 95% confidence intervals will be reported. Model assumptions will be tested and adjusted if necessary. |
Inclusion Criteria:
Exclusion Criteria:
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The study involved 431 patients with colorectal cancer liver metastasis, all undergoing surgery at the Sixth Affiliated Hospital of Sun Yat-sen University. The cohort consisted of 297 patients in the training set and 104 patients in the testing set.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ethics Committee of the Sixth Affiliated Hospital of Sun Yat-sen University | Guangzhou | Guangdong | 510655 | China |
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This study recruited 431 colorectal cancer liver metastasis patients who underwent surgery at the Sixth Affiliated Hospital of Sun Yat-sen University (SAHSYSU), with liver metastasis samples from the hospital's archives. The training dataset included 1,994 whole slide images (WSIs) from 297 patients (batch dated July 3, 2013), and the testing dataset contained 972 WSIs from 104 patients (batch dated April 21, 2023). In 2024, two prospective experiments were conducted with 114 WSIs from 30 patients. One experiment involved a human-AI competition, where nine pathologists and AI independently interpreted WSIs for binary and quaternary classifications. The other assessed AI-assisted classification, where nine pathologists used AI support for the same tasks. Both experiments aimed to evaluate the model's performance and clinical applicability.
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| 6 months post-surgery (for prospective cohort) |
| Up to 3 years post-surgery |
| Overall Survival (OS, in months) in Colorectal Cancer Liver Metastasis (CRLM) Patients Stratified by AI-based Histopathological Growth Pattern (HGP) Classification | This outcome evaluates the association between AI-based HGP classification (desmoplastic and non-desmoplastic) and overall survival (OS) in patients with colorectal cancer liver metastasis (CRLM) following curative-intent resection. OS is defined as the time from surgery to death from any cause. Kaplan-Meier analysis will be used to estimate OS for each HGP group, with comparisons by log-rank test. Multivariate Cox regression models will assess the prognostic value of HGPs, adjusting for clinical covariates (e.g., age, sex, metastasis number/size, chemotherapy, margin status, tumor burden score). Hazard ratios with 95% confidence intervals will be reported. Model assumptions will be tested and adjusted if necessary. | Up to 3 years post-surgery |
| Time to Diagnosis (in minutes) by Pathologists With and Without AI-Assisted COFFEE Model in CRLM HGP Classification | This outcome assesses the impact of the AI-assisted COFFEE model on diagnostic efficiency by comparing the time required by pathologists to classify histopathological growth patterns (HGPs) of colorectal cancer liver metastasis (CRLM), with and without COFFEE assistance. The metric is the time (minutes) from slide review start to final diagnosis, measured for each pathologist using a standardized digital whole slide image platform. The comparison includes two arms: the AI-assisted diagnosis arm, where junior pathologists use COFFEE as a decision-support tool, and the conventional diagnosis arm, where pathologists perform manual classification based on visual histopathological assessment. All participants review the same set of slides in randomized order, and diagnostic time is logged by the viewing software. Descriptive statistics (median, IQR) will be reported. | During the prospective trial period (6 months) |
| Diagnostic Accuracy (percentage of correct classifications) of Pathologists With and Without AI-Assisted COFFEE Model in CRLM HGP Classification | This outcome evaluates the diagnostic accuracy of pathologists in classifying histopathological growth patterns (HGPs) of colorectal cancer liver metastasis (CRLM), comparing AI-assisted versus conventional diagnostic workflows. Accuracy is defined as the proportion of correctly classified whole slide images (WSIs) relative to a gold-standard consensus diagnosis by expert gastrointestinal pathologists. Each pathologist will independently classify the same set of CRLM WSIs under two conditions: with AI assistance (COFFEE model) and without AI assistance (manual assessment). Classification will be evaluated for both binary HGP categories (desmoplastic vs. non-desmoplastic) and four-class HGP categories (desmoplastic, replacement, pushing, mixed). Accuracy will be calculated as: Accuracy = Total number of predictions / Number of correct predictions×100%. | During the prospective trial period (6 months) |