The primary aim of this study is to assess the effect of EDP-323 on the pharmacokinetics and safety of midazolam, caffeine, and rosuvastatin in healthy adult participants.

Inclusion Criteria:

• An informed consent document signed and dated by the subject
• Healthy male and female subjects of any ethnic origin between the ages of 18 and 65 years, inclusive
• Screening body weight >50 kg and body mass index (BMI) of 18 to 32 kg/m2
• Female subjects of childbearing potential must agree to use two effective methods of contraception from the date of Screening until 30 days after the last dose of EDP-323. A male participant who has not had a vasectomy and is sexually active with a woman of childbearing potential must agree to use effective contraception from the date of Screening to 90 days after his last dose of study drug.

Exclusion Criteria:

• Clinically relevant evidence or history of illness or disease
• Pregnant or nursing females
• History of febrile illness within 7 days prior to the first dose of study drug or subjects with evidence of active infection
• Any condition possibly affecting drug absorption (e.g., gastrectomy, cholecystectomy)
• Positive for HIV or hepatitis
• Positive urine drug screen
• Current tobacco smokers or use of tobacco within 3 months prior to Screening.
• Received any vaccine, an investigational agent, or biological product within 28 days or 5 times the t½ of receipt of study drug, whichever is longer.
• Drug sensitivity to midazolam, caffeine, rosuvastatin