The experimental group went through a fatigue protocol, in which speed and incline increase one unit each minute passing, and stopped when rate of perceived exertion level reached ≥ 18, and the plasma lactate level differed from the baseline by ≥ 4mmol/L

Will rest for 20 minutes

The experimental group went through a fatigue protocol, in which speed and incline increase one unit each minute passing, and stopped when rate of perceived exertion level reached ≥ 18, and the plasma lactate level differed from the baseline by ≥ 4mmol/L.

Dynamic postural balance was measured using Y-balance (YB). A test designed to examine the ability to maintain postural stability in dynamic conditions. In this test, participants would single-leg stand on a block (start with left) and were instructed to push another block with their other leg as far as they can without losing their balance (i.e. placing their leg on the ground), while having their hands on their hips. The free blocks are placed anteriorly and posterolaterally of the participant in both directions, creating a Y shape with the stable block on the intersection of these lines. Distance reached was recorded for each direction. The directions of the test were anterior, posteromedial, posterolateral (pushing on the contralateral side). Then, the participants were asked to switch their feet and preform the task again; six trials in total were recorded to minimize learning effect.

Static balance (SB) testing was performed using virtual reality interactive gaming system (Wii balance board (WBB) [Nintendo, Kyoto, Japan]). The WBB contains four strain gauge-based load sensors, qualified to obtain center of pressure movement, which makes it valid and reliable to measure posture stability5. The participants were asked to perform stand quietly with their hand on their sides with both legs with equal weight-bearing and on a single-leg stance on the right leg, then on the left leg. All postures were performed under two conditions: open eyes and closed eyes.