This prospective interventional study investigates the effects of a prostaglandin analog-based eyelash serum on ocular surface integrity, tear film stability, and eyelash growth over six weeks. Nineteen healthy female participants applied the serum daily, with clinical assessments performed at baseline and post-treatment. The study adhered to ethical guidelines, and parameters such as tear film stability, tear volume, ocular hyperemia, and eyelash characteristics were evaluated.

Inclusion Criteria:

• Female individuals aged 18 to 30 years.
• Corrected distance visual acuity (CDVA) of 0.10 LogMAR or better in both eyes.
• Fitzpatrick skin type III or lower.
• No active dermatological conditions affecting the periocular area.
• Regular use of cosmetic products (e.g., mascara, eyeliner, or eyeshadow) with no history of intolerance.
• Willingness and ability to follow the serum application protocol for six weeks.

Exclusion Criteria:

• History or diagnosis of dry eye disease or other ocular surface disorders.
• Previous ocular surgery, trauma, or systemic conditions affecting ocular surface function.
• Use of topical or systemic medications that could impact tear film stability.
• Documented hypersensitivity or allergic reactions to cosmetic or ophthalmic products.
• Presence of eyelid disorders that could interfere with eyelash growth (e.g., blepharitis, trichiasis).
• Use of growth-enhancing serums within the past three months.
• Pregnancy or breastfeeding at the time of enrollment.

Individual participant data (IPD) will not be shared due to privacy concerns and ethical considerations. The collected data include sensitive ocular health parameters, and sharing such data could compromise participant confidentiality. However, aggregated data and study results will be available upon reasonable request for academic and research purposes.