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| ID | Type | Description | Link |
|---|---|---|---|
| AWEEC/BAU/2024(2)/15(a) | Other Identifier | Animal Welfare and Experimentation Ethics Committee, Bangladesh Agricultural University, Mymensingh 2202 |
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This study will employ a randomized controlled intervention design to evaluate the health impacts of consuming fresh-cut street-vended fruits, focusing on microbial contamination and gastrointestinal (GI) symptoms. A total of 300 participants will be recruited and divided into treatment (consuming guava, pineapple, or watermelon) and control groups (no fruit consumption). Fruit samples will be analyzed for microbial contamination, including S. aureus and E. coli, using standard microbiological and molecular techniques. Data on GI symptoms will be collected through questionnaires and analyzed using statistical methods, such as Chi-square tests, Kaplan-Meier survival analysis, and Cox proportional hazards regression. Spearman correlation will identify associations between bacterial presence and specific symptoms. Ethical approval will be obtained, and participant safety will be prioritized. Analytical tools, including IBM SPSS, RStudio, and PyCharm, will be utilized for the analyses.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control Group (No Fresh-Cut Fruit Consumption) | No Intervention | Participants in this group did not consume any street-vended fresh-cut fruits. They continued their usual dietary habits, avoiding any foods that could potentially cause irritation or foodborne illness. This group served as a baseline for comparison against the treatment groups. | |
| Fresh-Cut Fruit Consumers with Gastric Acidity (Guava) | Experimental | Participants with pre-existing gastric acidity conditions consumed fresh-cut guava from street vendors. Their health status was monitored for five days post-consumption, focusing on gastrointestinal (GI) symptoms and microbial contamination effects. |
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| Fresh-Cut Fruit Consumers with Gastric Acidity (Pineapple) | Experimental | Participants with pre-existing gastric acidity conditions consumed fresh-cut pineapple from street vendors. Post-consumption symptoms and microbial contamination were assessed over a five-day period. |
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| Fresh-Cut Fruit Consumers with Gastric Acidity (Watermelon) | Experimental | Participants with pre-existing gastric acidity conditions consumed fresh-cut watermelon from street vendors. GI symptoms and potential microbial contamination effects were monitored for five days. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Fresh-Cut Guava Consumption | Dietary Supplement | Participants eat a defined portion of guava. |
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| Measure | Description | Time Frame |
|---|---|---|
| Prevalence of Gastrointestinal Symptoms Among Participants After Consumption of Fresh-Cut Fruits | From enrollment to the end of treatment at 10 days |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Md. Ariful Islam, PhD | Bangladesh Agricultural University, Mymensingh | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Microbiology and Hygiene, Bangladesh Agricultural University | Mymensingh | 2202 | Bangladesh |
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This study employs a randomized controlled intervention design with a parallel assignment model to evaluate the health impacts of consuming fresh-cut street-vended fruits, focusing on microbial contamination and gastrointestinal (GI) symptoms. A total of 300 participants will be assigned to one of seven arms: two treatment arms with 75 participants each (one with gastric acidity problems and one without), subdivided into three subgroups based on the type of fruit consumed (guava, pineapple, and watermelon). Additionally, a control group of 75 participants will not consume any fresh-cut street-vended fruits and will follow their usual dietary habits. The primary aim is to assess the microbial load (Total Viable Count, S. aureus, and E. coli) on fruits and its association with the development of GI symptoms. The study will employ statistical analysis techniques, including chi-square tests, relative risk analysis, and correlation studies, to evaluate the impact of fruit consumption on
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| Fresh-Cut Fruit Consumers without Gastric Acidity (Guava) | Experimental | Participants without pre-existing gastric acidity conditions consumed fresh-cut guava from street vendors. Health status was tracked for five days, with a focus on microbial contamination and foodborne illness symptoms. |
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| Fresh-Cut Fruit Consumers without Gastric Acidity (Pineapple) | Experimental | Participants without pre-existing gastric acidity conditions consumed fresh-cut pineapple from street vendors. The study monitored their health for five days to evaluate any adverse effects. |
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| Fresh-Cut Fruit Consumers without Gastric Acidity (Watermelon) | Experimental | Participants without pre-existing gastric acidity conditions consumed fresh-cut watermelon from street vendors. GI symptoms and microbial contamination effects were assessed over five days. |
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| Fresh-Cut Pineapple Consumption | Dietary Supplement | Participants consumed fresh-cut pineapple purchased from street vendors. Health outcomes, including potential microbial contamination and GI symptoms, were assessed for five days after consumption. |
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| Fresh-Cut Watermelon Consumption | Dietary Supplement | Fresh-Cut Watermelon Consumption Description: Participants consumed fresh-cut watermelon obtained from street vendors. GI symptoms and microbial contamination effects were monitored for five days following consumption. |
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| Fresh-Cut Guava Consumption (With Gastric Acidity) | Dietary Supplement | Same as intervention 2, but in participants with acidity |
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| Fresh-Cut Pineapple Consumption (With Gastric Acidity) | Dietary Supplement | Same as intervention 3, but in participants with acidity. |
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| Fresh-Cut Watermelon Consumption (With Gastric Acidity) | Dietary Supplement | Same as intervention 4, but in participants with acidity. |
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| ID | Term |
|---|---|
| D005767 | Gastrointestinal Diseases |
| D005517 | Foodborne Diseases |
| D013203 | Staphylococcal Infections |
| D004927 | Escherichia coli Infections |
| ID | Term |
|---|---|
| D004066 | Digestive System Diseases |
| D011041 | Poisoning |
| D064419 | Chemically-Induced Disorders |
| D016908 | Gram-Positive Bacterial Infections |
| D001424 | Bacterial Infections |
| D001423 | Bacterial Infections and Mycoses |
| D007239 | Infections |
| D004756 | Enterobacteriaceae Infections |
| D016905 | Gram-Negative Bacterial Infections |
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