This study investigates the effects of three polishing methods using a bristle brush, rubber cup, and air polishing on the surface roughness of scaled human teeth. The study aims to determine which polishing method is most effective in minimizing roughness and preventing plaque accumulation, thus contributing to improved oral health practices.

Tooth surface roughness influences plaque retention and periodontal health. Polishing after scaling helps smoothen enamel surfaces, reducing bacterial adhesion. While various studies have assessed polishing techniques, there are inconsistencies in findings, particularly regarding air polishing vs. rubber cup polishing. Additionally, most studies focus on bovine teeth or indirect restorative materials rather than extracted human teeth. Using a randomized controlled trial (RCT) design, 56 extracted teeth will be divided into four groups: a control group (no polishing) and three experimental groups subjected to different polishing techniques. Surface roughness will be analyzed using a Scanning Electron Microscope (SEM). This study aims to fill these research gaps and provide region-specific data by determining which polishing method is most effective in minimizing roughness and preventing plaque accumulation, thus contributing to improved oral health practices.

Inclusion Criteria:

• Extracted human teeth with intact buccal or lingual surfaces.
• Patients with a history of trauma affecting the oral cavity will be eligible, provided they meet other criteria (e.g., intact buccal or lingual surfaces).
• Male and female patients aged 18 years and above.

Exclusion Criteria:

• Teeth with restorations, caries, fractures, or significant developmental defects (e.g., amelogenesis imperfecta, dentinogenesis imperfecta , fluorosis etc).
• Patients with systemic disease such as diabetes mellitus, cardiovascular disorders or any other condition will be excluded.
• Mentally impaired patient.
• Patients who have undergone scaling and polishing within the last 3 months.

The study will share de-identified individual participant data (IPD), including surface roughness measurements obtained from Scanning Electron Microscopy (SEM) analysis, as well as statistical findings related to different polishing techniques. The shared dataset will help researchers conduct secondary analyses and compare results with similar studies.

The data will be made available 6 months after the study's completion and will remain accessible for a period of 5 years to facilitate further research.

Access to the data will be granted to qualified researchers upon submission of a formal request that includes a research proposal outlining the intended use of the data. Requests will be reviewed by the study investigators and ethical review committee to ensure compliance with data privacy regulations.

This study follows a double-blind approach where both the SEM technician capturing micrographs and the analyst evaluating surface roughness will be blinded to the specific group allocations.