30 participants underwent a 16-week program of aerobic (60%-70% HRmax for 40 minutes) and resistance exercises (65%-70% RM for 30 minutes).

30 participants maintained normal physical activity without additional interventions.

Participants are required to perform aerobic and resistance exercises. Aerobic exercises included the use of treadmills and elliptical machines, with intensity maintained at 60%-70% of the maximum heart rate (calculated as 220 minus age), for a total of 40 minutes per session (5 minutes of warm-up, 30 minutes of main exercise, and 5 minutes of cool-down). Heart rate monitors were used to ensure the intensity was maintained; resistance exercises involved gym equipment and bodyweight exercises, including the use of a pec deck machine, lat pull-downs, chest presses, bicep curls, tricep extensions, leg curls, and leg presses. The intensity of resistance exercises was set at about 65%-70% of one repetition maximum (1RM), with each session consisting of three sets of 6-8 repetitions and lasting for 30 minutes.

Autonomic function was assessed using the Pushikang FLY-2 autonomic physiological monitor. Participants were required to abstain from caffeinated and alcoholic beverages for 12 hours and from strenuous physical activity for 48 hours prior to the test. The testing was conducted between 8:00 a.m. and 11:00 a.m. in a controlled environment with temperatures maintained at 22-24°C. The test duration was 5 minutes, during which participants sat quietly and refrained from speaking. Autonomic nerve function parameters were recorded using finger photoplethysmography to measure pulse wave changes in a resting state. Data was processed using Beijing Fourier's autonomic nerve response analysis software (ANS-1, V6.6). Time-domain analysis focused on the mean interval between heartbeats to reflect sympathetic/vagal modulation, and the variance of these intervals indicated the overall variability of cardiac function, providing critical prognostic information.

Cardiopulmonary function was evaluated using the cardiopulmonary exercise test (CPET, Schiller) on a Rehab-B exercise treadmill. To ensure test accuracy, participants were instructed to refrain from physical activity on the day prior to the test and to avoid alcohol, caffeinated beverages, and food for 8 hours before testing and during the first 3 hours of the test day. The exercise commenced with a 3-minute rest followed by a gradual load phase, allowing participants to reach voluntary fatigue within 8 to 12 minutes, and then continue at maximum tolerance for an additional 3 minutes. The exercise protocol followed the Bruce protocol, with incremental loads adjusted for gender: 25 W/min for males and 15 W/min for females.

The Functional Movement Screening (FMS) is a standardized tool used to assess the functional motor abilities of individuals. It consists of seven tests (squatting, stepping, lunging, reaching, leg raising, push-up, and rotary stability) that balance flexibility and stability, allowing medical and fitness professionals to identify problems such as weakness, imbalance, asymmetry, and movement limitations. In the FMS, each action is scored from 0 to 3: a score of 3 indicates the individual can perform the movement standardly and stably without compensation; a score of 2 signifies that the movement can be completed, albeit with some instability or compensatory behavior; a score of 1 indicates an inability to perform the movement according to the standards. Research has shown that lower FMS scores correlate with a higher risk of injury. Typically, a threshold score of 14 is used, with individuals scoring below this level considered at greater risk of injury.