In the research, the investigators will monitor the bacterial colonization of nails coated with a methacrylic coating

It is known that the hand hygiene of healthcare workers is extremely important in order to prevent health-related infections. The effectiveness of hand disinfection can be affected by aspects of hand hygiene such as long nails and fingernail applications. In order to determine whether and to what extent fingernails applied among healthcare workers pose a risk of transmission of potentially pathogenic bacteria, and until what day artificial fingernails are safe in healthcare workers, the investigators will monitor bacterial colonization on nails with UV-cured methacrylic fingernails lamp and compared it with a control group of natural nails without application.

Inclusion Criteria:

• nurses, doctors and respiratory physiotherapists employed in high-risk wards for patients
• healthy nail surface and cuticle

Exclusion Criteria:

• subjects with damaged nails or cuticles
• operating room staff using surgical hand brushing and staff in general nursing wards.