In addition to collecting product residues, dirt and oil, makeup products can damage the skin and cause infection as they are a breeding ground for bacteria. Studies investigating the microbiological contamination of makeup applicators have shown that makeup sponges and brushes are a means of transmitting pathogens. Other studies have determined that positive isolates are detected in almost every device taken from makeup tools. In order to draw attention to the source of infection that is not widely known by the public and to instill correct hygiene habits, this study aims to detect pathogenic bacteria in makeup tools used by university students and to evaluate the effectiveness of the hygiene training provided.

Makeup does a good job of improving the appearance of women. However, many women do not know that makeup can be a health hazard because it contains bacteria and can spread infection. In addition to collecting product residue, dirt, and oil, makeup products can damage the skin and cause infection because they are a breeding ground for bacteria. Cosmetic contamination leads to various types of infections, ranging from mild to serious.

Studies investigating the microbiological contamination of used makeup applicators have shown that makeup sponges and brushes are a vehicle for pathogen transmission. Other studies have determined that Staphylococcus aureus grows as the dominant bacteria in makeup tools and that positive isolates are detected in almost every device taken. The American Academy of Dermatology recommends washing your makeup brushes every 7 to 10 days to protect your skin and kill any harmful bacteria left on your makeup brushes.

Infectious diseases are still a major public health problem, especially in developing countries. Therefore, it is important for people to adopt proper hygiene habits, especially hand washing, to prevent infection. In order to draw attention to a source of infection that is not widely known by the public and to instill correct hygiene habits, this study aims to detect pathogenic bacteria in make-up tools used by university students and to evaluate the effectiveness of the hygiene education provided.

Inclusion Criteria:

• Being registered at Ufuk University School of Nursing,
• Being over 18 and under 30
• Being female,
• Having make-up tools,
• Being volunteer to participate in the research.

Exclusion Criteria:

• Being male,
• Not having make-up tools,
• Giving up participating in the research,
• Under 18 years old, over 30 years old

participants and outcome assessors will not be informed about group assignments and will be blinded.