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Until now, cancer is still associated with high morbidity and mortality, and is therefore a big issue for the public health. Colorectal cancer is the second leading cause of cancer death in developed countries. In advanced colorectal cancer, 60% of these patients develop cachexia. The presence of cachexia plays an important role in the high mortality rate of cancer. Cachexia is characterized by involuntary weight loss with loss of skeletal muscle with or without loss of fat mass.
The aim of this study is to determine the feasibility of taking muscle biopsies of cachectic colorectal cancer patients. Skeletal muscle characteristics will be compared between biopsies of the m. erector spinae and the m. vastus lateralis from cachectic colorectal cancer patients, non-cachectic colorectal cancer patients and healthy controls.
10 cachectic, 25 non-cachectic, and 25 healthy controls will be recruited for this study. It is an observational study, so measurements will be taken at one time point. Interventions/measurements:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Cachectic colorectal cancer patients |
| ||
| Non-cachectic colorectal cancer patients |
| ||
| Healthy controls |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Minimal invasive fine needle muscle biopsies | Procedure | Fine needle muscle biopsies will be taken from the m. erector spinae and the m. vastus lateralis. Participants are placed in a standardized prone position on the examination table for measurements of the m. erector spinae and in a supine position for measurements of the m. vastus lateralis. |
| Measure | Description | Time Frame |
|---|---|---|
| Skeletal muscle fiber typing | Immunofluorescence staining of skeletal muscle biopsies to observe different muscle fiber types (myosin heavy chains) | at enrollment |
| skeletal muscle nuclei | Immunofluorescence staining with DAPI | at enrollment |
| Inflammatory parameters | ELISA (bloodsamples), immunofluorescence staining (muscle biopsies) | at enrollment |
| Mitochondrial morphology | mitochondrial fusion and fission (western blot) mitochondrial OXPHOS (western blot) | at enrollment |
| Measure | Description | Time Frame |
|---|---|---|
| 3D freehand ultrasound | measuring the volume of the m. rectus femoris | at enrollment |
| CT-scans | level lumbar 3 To calculate the cross-sectional area of the m. erector spinae. |
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Cachectic colorectal cancer patients.
Inclusion criteria:
Exclusion Criteria:
Non-Cachectic cancer patients.
Inclusion criteria:
Exclusion criteria:
Healthy controls.
Inclusion criteria:
- minimum age of 18 years old
Exclusion criteria:
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12 cachectic colorectal cancer patients, 25 non-cachectic colorectal cancer patients, and 25 healthy controls will be recruited in the study. Colorectal cancer patients will be recruited by the surgeans from the Jessa Hospital (Hasselt, Belgium), and the healthy controls will be recruited by the local researcher from Hasselt University.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Britt van de Haterd, PhD student | Contact | 0032479871509 | britt.vandehaterd@uhasselt.be | |
| Kenneth Verboven, Professor | Contact | kenneth.verboven@uhasselt.be |
| Name | Affiliation | Role |
|---|---|---|
| Kenneth Verboven, Professor | Hasselt University - REVAL | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hasselt University - REVAL research institution | Recruiting | Diepenbeek | Limburg | 3520 | Belgium |
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| ID | Term |
|---|---|
| D015179 | Colorectal Neoplasms |
| D002100 | Cachexia |
| ID | Term |
|---|---|
| D007414 | Intestinal Neoplasms |
| D005770 | Gastrointestinal Neoplasms |
| D004067 | Digestive System Neoplasms |
| D009371 | Neoplasms by Site |
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|
| at enrollment |
| Activity pattern | PASIPD questionnaire | at enrollment |
| VAS score | to descripe the pain at the moment of the biopsy procedure. Score range is 0 to 10. With 0 no pain until 10 the worst pain. | at enrollment |
| Handgrip strength | Using the JAMAR to measure the handgripstrength | at enrollment |
| Short Physical Performance Battery (SPPB) test | Including a balance test, sit-to-stand test and four meter walking test | at enrollment |
| Quality of Life questionnaire | SF-36 questionnaire 36-item patient-reported questionnaire that covers eight health domains: physical functioning (10 items), bodily pain (2 items), role limitations due to physical health problems (4 items), role limitations due to personal or emotional problems (4 items), emotional well-being (5 items), social functioning (2 items), energy/fatigue (4 items), and general health perceptions (5 items). Scores for each domain range from 0 to 100, with a higher score defining a more favorable health state. | at enrollment |
| skeletal muscle capillarization | Immunofluorescence staining for capillaries in the skeletel muscle biopsies. | at enrollment |
| Jessa Hospital | Recruiting | Hasselt | Limburg | 3500 | Belgium |
|
| D009369 | Neoplasms |
| D004066 | Digestive System Diseases |
| D005767 | Gastrointestinal Diseases |
| D003108 | Colonic Diseases |
| D007410 | Intestinal Diseases |
| D012002 | Rectal Diseases |
| D015431 | Weight Loss |
| D001836 | Body Weight Changes |
| D001835 | Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D013851 | Thinness |