A randomized controlled open-label trial will be conducted in Mexico City, recruiting healthy participants who do not consume excessive alcohol or smoke. A selection visit will be conducted during which blood and urine samples will be collected, and assessments of diet, anthropometric measurements, and body composition will be performed.

A randomized controlled open-label trial will be conducted in Mexico City, recruiting healthy participants who do not consume excessive alcohol or smoke. A selection visit will be conducted during which blood and urine samples will be collected, and assessments of diet, anthropometric measurements, and body composition will be performed.

Participants will be randomized into three groups for the intake of meals containing the foods of interest and control without these foods. After the meal consumption including foods of interest, a 24-hour urine sample will be collected by the participants. Urine and blood samples will be analyzed using using untargeted metabolomics in different platforms to determine biomarker concentrations.

Inclusion Criteria:

• Age 18 years and older
• Body mass index (BMI ) between 18.5 to 30 kg/m2
• Not having allergies to the study foods (ataulfo mango, nopal, guava, avocado, corn tortilla and amaranth).
• Without consumption of dietary supplements at the time of participating in the study.
• Blood chemistry within reference values (glucose <126 mg/dL, triglycerides <250 mg/dL, total cholesterol <250 mg/dL.

Exclusion Criteria:

• The exclusion criteria were participants with any other chronic disease diagnosed by any doctor.
• Were pregnant.
• Smoking habit durig the trial.
• Intake of alcoholic beverages during the trial.