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| Name | Class |
|---|---|
| Karolinska Institutet | OTHER |
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The overall goal is to describe the oxidation state of albumin before, during and after liver transplantation. This is a pilot observational study in which the oxidative state in 10 patients undergoing liver transplantation will be described. Samples for analysing the oxidation state of albumin, general oxidative damage and anti-oxidant state, the activation of the complement system and factors involved in coagulation will be obtained before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30.
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| Measure | Description | Time Frame |
|---|---|---|
| Albumin quality | Describe potential qualitative changes in albumin during the liver transplantation procedure. Non-oxidised, single oxidised and double oxidised albumin will be analysed by HPLC. | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Measure | Description | Time Frame |
|---|---|---|
| Effect of clinical factors on albumin quality before transplantation | Explore the impact of different clinical factors on albumin's oxidative state before transplantation. For this a multiple regression model will be used including clinical parameters; sex (male or female), age (years), and MELD (Model for end-stage liver disease). | Before transplantation. |
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Inclusion Criteria:
Exclusion Criteria:
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Patient that are accepted for liver transplantation and put on the waiting list will be included
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Olav Rooijackers, PhD | Contact | 0739661645 | olav.rooyackers@ki.se | |
| Håkon Haugaa, MD, PhD | Contact | hokan.haugaa@regionstockholm.se |
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Data might be shared on special request of researchers
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| ID | Term |
|---|---|
| D017093 | Liver Failure |
| D008103 | Liver Cirrhosis |
| D013927 | Thrombosis |
| ID | Term |
|---|---|
| D048550 | Hepatic Insufficiency |
| D008107 | Liver Diseases |
| D004066 | Digestive System Diseases |
| D005355 | Fibrosis |
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Plasma and serum samples will be obtained
| Effect of clinical factors on albumin quality after transplantation | Explore the impact of different clinical factors on albumin's oxidative state after transplantation. For this a multiple regression model will be used including clinical parameters; sex (male or female), age (years), MELD (Model for end-stage liver disease), bleeding during surgery (in liters), time of surgery (hours). | On postop days 1, 2 and about 30. |
| Graft outcome in relation to albumin quality | Explore of the oxidation state of albumin (percentage of albumin that is oxidised) is correlated with graft function (bilirubin levels in mg/L) | At days 1, 2 and about 30 days after transplantation |
| Oxidative stress in relation to albumin quality | Explore is the oxidation state of albumin (percentage of albumin oxidised) is related to a general oxidative state measured by TBARS (Thiobarbituric acid reactive substances) measured as MDA (malondialdehydes) in nmol/ml. | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Anti-oxidant capacity in relation to albumin quality | Explore is the oxidation state of albumin (percentage of albumin oxidised) is related to the total anti-oxidant capacity measured a the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using a phenanthroline substance. | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Binding capacity | Explore whether the oxidation state of albumin (percentage of albumin oxidised) is affecting albumin's binding capacity measured by the ability of albumin in the sample to bind cobalt (mg/ml). | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Complement system | Explore how the complement system (C3bc in mg/ml) is affected during liver transplantation and how this is related to albumin quality (percentage of albumin oxidised) | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Complement system | Explore how the complement system (terminal complement complex in mg/ml) is affected during liver transplantation and how this is related to albumin quality (percentage of albumin oxidised) | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| Coagulation | Explore how coagulation (Thrombin-antithrombin complex in microgram/ml) is affected during liver transplantation and how this is related to albumin quality (percentage albumin oxidised) | Before transplantation, before and after reperfusion of the portal vein and on postop days 1, 2 and about 30. |
| D010335 |
| Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D016769 | Embolism and Thrombosis |
| D014652 | Vascular Diseases |
| D002318 | Cardiovascular Diseases |