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| ID | Type | Description | Link |
|---|---|---|---|
| Company Sponsored | Other Identifier | Acorn Biolabs |
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To explore the application of cells expanded from plucked hair follicles after collection, transport, and cryopreservation, in disease modeling and cell-based therapies.
Hair Follicles collected and transported in Acorn's proprietary transport media can serve has therapeutic starting materials for future cell-based therapeutics. This study is to characterize primary cell outgrowth, functional stem cell generation and induced pluripotent stem cell line(s) from primary outgrowth(s) as well as evaluate and characterize differentiation of stem cell lines into functional cell types required for various disease states and cell-based application(s).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Hair Follicle Donors | Healthy individuals, aged 18-80, who will have hair follicles collected |
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| Measure | Description | Time Frame |
|---|---|---|
| Characterize primary cellular outgrowth(s) | 10 hair follicles will be placed in a tissue culture plate per participants. Over the course of 30 days, the outgrowth percentage (the number of outgrowths present out of 10 will be monitored and the confluency (percent of cells present in relation to surface area) will be noted for each participant. | 30 days |
| Evaluate functional stem cell generation and induced pluripotent stem cell line(s) from primary outgrowth(s) | 1 million keratinocytes will be taken for reprogramming for each participant. The number of iPS colonies observed on day 21 will be noted, resulting in a reprogramming efficiency score. | 21 days |
| Evaluate and characterize differentiation of stem cell lines into functional cell types required for various disease states and cell-based application(s) | iPS cell lines which have been confirmed to be pluripotent will be differentiated into pancreatic progenitor cells and hematopoietic stem cells. The cells will be analyzed using FLOW cytometry and the percentage of double positive cells will be noted for each participant. This will be repeated for multiple pathways such as the hematopoietic and endothelial lineage(s). | 30 days |
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Inclusion Criteria:
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Healthy population, aged 18-80, without scalp or hair disorders, chronic illnesses that may impact scalp health or wound healing, and not on immunosuppressants, steroids, or medications that cause hair loss.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Drew Taylor, MSc, PhD | Contact | 226-929-8476 | info@acorn.me |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Acorn Biolabs | Recruiting | Toronto | Quebec | M5R 0C7 | Canada |
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| ID | Term |
|---|---|
| D003920 | Diabetes Mellitus |
| D014947 | Wounds and Injuries |
| ID | Term |
|---|---|
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D004700 | Endocrine System Diseases |
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hair follicles