Background: To investigate the effect of molar incisor hypomineralization (MIH) on periodontal health, to evaluate the relationship between gingival crevicular fluid (GCF) volume, and GCF biomarkers such as matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-20 (MMP-20), and transforming growth factor-beta 1 (TGF-β1), and MIH.

Methods: The study was conducted on 40 pediatric patients diagnosed with MIH (Study Group/SG), and 40 pediatric patients healthy for MIH (Control Group/CG). Clinical periodontal measurements (plaque index (PI), gingival index (GI), probing pocket depth (PPD), and GCF volume were performed. Concentrations of MMP-8, MMP-20, and TGF-β1 in GCF were determined using enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U test and T-test were used for comparisons between groups. Significance was set at p=0.05 for statistical analysis.

Inclusion Criteria:

• the ages of 8 and 14 who were diagnosed with MIH in their maxillary permanent first molars,
• had healthy maxillary permanent first molars,
• scored 3 (positive) or 4 (definitely positive) on the Frankl Behavior Scale,
• had no mental or physical disability,
• did not need urgent treatment and/or were not in pain,
• agreed to participate in the study with their parents and signed the consent form.

Exclusion Criteria:

• Children with systemic, chronic, and/ or metabolic diseases,
• under active orthodontic treatment, with developmental defects of enamel (excluding MIH), or born prematurely

Pediatric population with MIH