The patient awaited allogeneic corneal donation and underwent corneal transplantation surgery.

The corneal limbal tissue (2mm x 5mm) was harvested from the patient's healthy eye, and ex vivo amplification was performed to obtain an adequate quantity of transplantable corneal limbal stem cells and corneal stromal stem cells.

The transplantation area for the cells is the ocular surface, so the primary measure of safety will focus on post-transplant ocular symptoms. These include eye irritation symptoms, conjunctival hyperemia, corneal surface edema, and anterior chamber flare. Adverse events are defined as follows: eye irritation symptoms scoring ≥1, conjunctival hyperemia scoring ≥2, corneal surface edema scoring ≥2, anterior chamber flare scoring ≥2, or a total score of 7 or above.

The healthy eye requires postoperative observation, and if infection or delayed healing occurs after tissue sampling, treatment will be necessary.

including cornea, conjunctiva, lens, vitreous body and retina. Score each indicator from 0 to 3 based on the standards in the table, and then calculate the final score.

Corneal microscopic structure examination. Observe whether the morphology of each layer of the cornea is normal.

Analyze the morphology and curvature characteristics of the entire corneal surface.

Observe the depth of corneal lesions to assess the condition and treatment efficacy.

record the statement of ocular surface

The VFQ-25 (Visual Function Questionnaire-25) is a widely used tool to assess the health-related quality of life related to visual function. It consists of 25 questions to evaluate how vision affects daily activities, emotional well-being, and social interactions. The questionnaire covers several domains: general vision, near and distance vision, social functioning, role difficulties, mental health, and driving. It is often used in clinical settings to track the progression of eye diseases, evaluate treatment outcomes, or compare the quality of life between different patient groups. A higher score indicates better visual function and quality of life, meaning the patient experiences fewer difficulties and less impairment in daily activities due to vision problems. Therefore, higher scores are better in this context.

Evaluate myocardial function through electrocardiography (ECG) and X-ray, focusing on T-wave morphology, QT interval duration, and ST segment changes. Specific findings are based on the hospital's ECG report. The cardiac silhouette should also be assessed for signs of heart failure or pericardial effusion in X-ray. A physician will determine their clinical significance if abnormalities are detected, considering factors such as correlation with the patient's medical history or whether the findings indicate an old lesion or non-pathological variation.

Under fasting conditions, conduct blood tests for complete blood count (CBC), blood biochemistry, coagulation function, pregnancy test (for female participants), and infectious disease screening. Results are evaluated against the normal reference ranges provided in the lab report. If abnormalities are detected, a physician determines their clinical significance. Hematologic testing aims to identify potential systemic adverse events, such as rejection or infection (e.g., elevated white blood cell count), and assess for infectious diseases (e.g., HIV, hepatitis B, syphilis).

Urinalysis. The results are assessed against the normal reference ranges provided in the lab report. If abnormalities are identified, a physician will determine their clinical significance.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

The head examination focuses on assessing the shape, symmetry, and condition of the scalp and facial features. Inspect for any deformities, scars, or abnormalities in the skull. Palpate for tenderness or masses, and observe for signs of trauma. The eyes should be examined for proper alignment, pupil size, and responsiveness. Check for conjunctival redness, discharge, or abnormalities. Inspect the nose for any deformities or discharge, and assess nasal patency. The ears should be examined for signs of infection, discharge, or abnormalities in shape and symmetry.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

The neck examination includes assessing the skin for any visible abnormalities such as scars or lumps. Palpate the neck for tenderness, masses, or enlarged lymph nodes. Examine the thyroid gland for enlargement, tenderness, or nodules. Assess the range of motion in the neck, and check for signs of pain, stiffness, or abnormal movement. Additionally, auscultate for any abnormal sounds, such as carotid bruits, that may indicate vascular abnormalities.

Palpate lymph nodes systematically, checking for size, consistency, mobility, and tenderness. Start with the cervical, axillary, and inguinal regions, then proceed to other areas if necessary. Normal lymph nodes should be small, mobile, and non-tender. Enlarged lymph nodes may suggest infection, inflammation, or malignancy. Assess their texture, whether soft or firm, and whether they are fixed or movable, as these characteristics can give clues about their underlying cause.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

For the spine, observe posture and alignment, and palpate along the cervical, thoracic, and lumbar regions for tenderness, deformities, or abnormal curvatures. Test for flexibility by asking the patient to bend, twist, or rotate the spine. For the limbs, inspect the joints and muscles for symmetry, swelling, or signs of injury. Palpate for warmth, tenderness, or deformities in the joints. Assess the range of motion in both upper and lower limbs, testing for strength, coordination, and any signs of weakness or numbness.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

The abdominal examination involves inspection, palpation, percussion, and auscultation. Start by observing the abdomen for signs of distension, scars, or abnormalities in contour. Palpate gently for tenderness, masses, or organ enlargement. Percuss to assess for fluid buildup or gas, and auscultate for bowel sounds. Pay attention to any signs of pain during palpation, particularly in the liver, spleen, or lower abdomen, which may indicate underlying pathology such as infections, tumors, or gastrointestinal issues.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

The neurological examination includes evaluating the patient's mental status, motor skills, coordination, and reflexes. Assess for any signs of cognitive impairment, speech difficulties, or mood changes. Test cranial nerve function, including vision, facial sensation, and strength. Evaluate motor function by observing muscle strength and tone, and test for signs of weakness or atrophy. Assess coordination with tasks such as finger-to-nose or heel-to-shin. Check for sensation abnormalities and perform reflex tests to assess the integrity of the central and peripheral nervous system.

The examination is conducted according to the standards of the "Diagnostic Medicine (10th Edition, People's Medical Publishing House)".

Examine the skin for color, texture, and any visible lesions, rashes, or scars. Look for signs of infection, redness, or swelling, and assess any pigmented lesions for irregularities in size, shape, or borders. Palpate the skin to check for moisture, temperature, and texture, noting any dryness, oiliness, or abnormalities. Inspect nails and hair for signs of systemic conditions. Look for bruising, ulcers, or signs of poor circulation, such as swelling or color changes in the extremities. The examination should also include a check for edema, especially in the lower limbs.